College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China.
College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China.
J Proteomics. 2018 Feb 20;173:139-154. doi: 10.1016/j.jprot.2017.12.007. Epub 2017 Dec 19.
Brassinosteroid is identified as an important hormone. However, information about brassinosteroid has not been fully elucidated, and few studies concerned its role in apple. The aim of this work was to study the role of brassinosteroid for apple tree growth. In our study, the effect of brassinosteroid on apple nursery tree was analyzed. The biomass, cell size and xylem content of apple nursery tree were obviously evaluated by brassinosteroid treatment; mineral elements contents, photosynthesis indexes, carbohydrate level and hormone contents were significantly high in brassinosteroid treated trees. To explore the molecular mechanisms of these phenotypic differences, iTRAQ-based quantitative proteomics were used to identify the expression profiles of proteins in apple nursery tree shoot tips in response to brassinosteroid at a key period (14days after brassinosteroid treatment). A total of 175 differentially expressed proteins were identified. They were mainly involved in chlorophyII biosynthesis, photosynthesis, carbohydrate metabolism, glycolysis, citric acid cycle, respiratory action, hormone signal, cell growth and ligin metabolism. The findings in this study indicate that brassinosteroid mediating apple nursery tree growth may be mainly through energy metabolism. Important biological processes identified here can be useful theoretical basis and provide new insights into the molecular mechanisms of brassinosteroid.
Brassinosteroid is very important for plant growth and development. However, the molecular mechanism of brassinosteroid mediating growth process is not perfectly clear in plant, especially in apple nursery tree. We used a combination of physiological and bioinformatics analysis to investigate the effects of brassinosteroid on apple nursery tree growth and development. The data reported here demonstrated that brassinosteroid regulates apple nursery tree growth mainly through energy metabolism. Therefore it can provide a theoretical basis from energy points for developing dwarfed or compact apple trees. This will benefit for low orchard management cost as well as early bearing, and high fruit yield as well as quality.
油菜素内酯被鉴定为一种重要的激素。然而，关于油菜素内酯的信息尚未完全阐明，并且很少有研究涉及它在苹果中的作用。本工作旨在研究油菜素内酯对苹果树生长的作用。在我们的研究中，分析了油菜素内酯对苹果苗圃树的影响。油菜素内酯处理明显评估了苹果苗圃树的生物量、细胞大小和木质部含量；油菜素内酯处理的树木中矿质元素含量、光合作用指标、碳水化合物水平和激素含量均显著升高。为了探索这些表型差异的分子机制，我们使用基于 iTRAQ 的定量蛋白质组学来鉴定油菜素内酯处理关键时期（油菜素内酯处理后 14 天）苹果苗圃树嫩梢中蛋白质的表达谱。共鉴定出 175 个差异表达蛋白。它们主要参与叶绿素生物合成、光合作用、碳水化合物代谢、糖酵解、柠檬酸循环、呼吸作用、激素信号、细胞生长和木质素代谢。本研究的结果表明，油菜素内酯介导苹果苗圃树生长可能主要通过能量代谢。这里确定的重要生物学过程可以作为有用的理论基础，并为油菜素内酯的分子机制提供新的见解。
油菜素内酯对植物的生长和发育非常重要。然而，油菜素内酯介导生长过程的分子机制在植物中并不完全清楚，尤其是在苹果苗圃树上。我们使用生理和生物信息学分析的组合来研究油菜素内酯对苹果苗圃树生长和发育的影响。这里报告的数据表明，油菜素内酯主要通过能量代谢调节苹果苗圃树的生长。因此，它可以从能量的角度为开发矮化或紧凑的苹果树提供理论依据。这将有利于降低果园管理成本以及早期结果、提高果实产量和品质。
