Pereira Luiz Miguel, de Luca Gabriela, Abichabki Nathália de Lima Martins, Bronzon da Costa Cássia Mariana, Yatsuda Ana Patrícia
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av do Café, sn/n, 14040-903, Ribeirão Preto, SP, Brazil; Núcleo de Apoio à Pesquisa em Produtos Naturais e Sintéticos, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av do Café, sn/n, 14040-903, Ribeirão Preto, SP, Brazil.
Vet Parasitol. 2018 Jan 15;249:92-97. doi: 10.1016/j.vetpar.2017.11.014. Epub 2017 Dec 2.
Neospora caninum is a member of Apicomplexa phylum, the causative agent of neosporosis. The neosporosis combat is not well established and several strategies related to vaccine, chemotherapy and immune modulation are under development. In this work, we evaluated the effects of artemisinin (Art), methylene blue (MB) and pyrimethamine (Pyr) alone or associated, on N. caninum proliferation and elimination using LacZ tagged tachyzoites. The reactive oxygen species (ROS) production after incubation with Art were also performed. Our results indicate that combinations of classical antimalarial drugs improve the parasite control, allowing the use of three drugs in a single dose. Additionally, artemisinin demonstrated distinct ROS production patterns in intra and extracellular N. caninum forms. The drug repurposing appears as a suitable approach, allowing a fast and safe method to evaluate old drugs but novel candidates against neosporosis.
犬新孢子虫是顶复门的一员，是新孢子虫病的病原体。对抗新孢子虫病的方法尚未完全确立，几种与疫苗、化疗和免疫调节相关的策略正在研发中。在这项工作中，我们使用 LacZ 标记的速殖子评估了青蒿素（Art）、亚甲蓝（MB）和乙胺嘧啶（Pyr）单独或联合使用对犬新孢子虫增殖和清除的影响。还检测了与青蒿素孵育后活性氧（ROS）的产生情况。我们的结果表明，经典抗疟药物的联合使用可改善对寄生虫的控制，使得三种药物可以单次给药。此外，青蒿素在细胞内和细胞外犬新孢子虫形态中表现出不同的 ROS 产生模式。药物重新利用似乎是一种合适的方法，它提供了一种快速且安全的方法来评估旧药，但却是对抗新孢子虫病的新候选药物。
