School of Materials Engineering, Neil Armstrong Hall of Engineering Purdue University, 701 West Stadium Avenue, West Lafayette, IN, 47907-2045, USA.
Small. 2018 Mar;14(10). doi: 10.1002/smll.201702828. Epub 2017 Dec 27.
The few lateral flow assays (LFAs) established for detecting the endocrine disrupting chemical bisphenol A (BPA) have employed citrate-stabilized gold nanoparticles (GNPs), which have inevitable limitations and instability issues. To address these limitations, a more stable and more sensitive biosensor is developed by designing strategies for modifying the surfaces of GNPs with polyethylene glycol and then testing their effectiveness and sensitivity toward BPA in an LFA. Without the application of any enhancement strategy, this modified BPA LFA can achieve a naked-eye limit of detection (LOD) of 0.8 ng mL , which is 12.5 times better than the LOD of regular BPA LFAs, and a quantitative LOD of 0.472 ng mL . This modified LFA has the potential to be applied to the detection of various antigens.
少数用于检测内分泌干扰化学物质双酚 A (BPA) 的侧向流动分析 (LFA) 采用了柠檬酸稳定的金纳米粒子 (GNP)，但这种方法存在不可避免的局限性和不稳定性问题。为了解决这些局限性，通过设计在 GNP 表面进行聚乙二醇修饰的策略，开发了一种更稳定、更灵敏的生物传感器，并在 LFA 中测试了它们对 BPA 的有效性和灵敏度。在不应用任何增强策略的情况下，这种改良的 BPA LFA 可以实现 0.8ng/mL 的肉眼检测限 (LOD)，比常规 BPA LFA 的 LOD 提高了 12.5 倍，定量 LOD 为 0.472ng/mL。这种改良的 LFA 有可能应用于各种抗原的检测。
