Department of Geratology, the First Affiliated Hospital to Nanjing Medical University, Nanjing, China.
Department of Anesthesia, the First Affiliated Hospital to Nanjing Medical University, Nanjing, China.
Obesity (Silver Spring). 2018 Feb;26(2):387-396. doi: 10.1002/oby.22104. Epub 2017 Dec 27.
Given the rising prevalence of central obesity and the discovery that beige cells appear within white adipose tissue, strategies to enhance these energy-expending adipocytes or "browning" within white adipose depots have become of therapeutic interest to combat obesity and its associated disorders. This study focused on, the role of microRNA (miRNA)-27b-3p in human visceral adipose tissue (VAT) browning.
Expression of miR-27b-3p and UCP1 in VAT and serum of humans was measured. MiR-27b-3p was overexpressed or suppressed in human visceral stromal fraction cells to analyze the potential role of miR-27b-3p.
UCP1 expression in human VAT decreased with elevated BMI and waist-hip ratio, whereas expression of miR-27b-3p was found to correlate positively with BMI and waist-hip ratio. High expression of miR-27b-3p was associated with reduced browning ability of human visceral adipocytes. Antagonism of miR-27b-3p led to the enhancement of browning ability in human visceral adipocytes.
These findings highlight the decreased browning ability of VAT from humans with obesity and the role of miR-27b-3p in regulating browning of human visceral adipocytes. They suggest that miR-27b-3p should be further explored as a potential target for the treatment of central obesity.
鉴于中心性肥胖的患病率不断上升，以及米色细胞出现在白色脂肪组织内的发现，增强这些消耗能量的脂肪细胞或“棕色化”白色脂肪组织的策略已成为治疗肥胖及其相关疾病的治疗靶点。本研究聚焦于 microRNA（miRNA）-27b-3p 在人类内脏脂肪组织（VAT）棕色化中的作用。
测量了 miR-27b-3p 和 UCP1 在人类 VAT 和血清中的表达。通过过表达或抑制人类内脏基质细胞中的 miR-27b-3p 来分析 miR-27b-3p 的潜在作用。
UCP1 在人类 VAT 中的表达随着 BMI 和腰臀比的升高而降低，而 miR-27b-3p 的表达与 BMI 和腰臀比呈正相关。miR-27b-3p 的高表达与人类内脏脂肪细胞的棕色化能力降低有关。miR-27b-3p 的拮抗作用导致人类内脏脂肪细胞的棕色化能力增强。
这些发现强调了肥胖人群 VAT 棕色化能力的降低，以及 miR-27b-3p 在调节人类内脏脂肪细胞棕色化中的作用。它们表明 miR-27b-3p 应该作为治疗中心性肥胖的潜在靶点进一步研究。
