Okamoto Eiryu, Miyanishi Hiroki, Nakamura Akinobu, Kobayashi Tomoyuki, Kobayashi Norihiko, Terawaki Yoshinori, Nagahata Hajime
Environmental Microbiology, College of Agriculture, Food and Environment Sciences, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
Cornes and Company Limited/Cornes Biogas, Sapporo Branch, Hokkaido, Japan.
Anim Sci J. 2018 Apr;89(4):727-732. doi: 10.1111/asj.12962. Epub 2017 Dec 27.
Changes in mastitis-causing pathogens, pH and water content in composted manure solids (CMS) prepared from digested slurry were evaluated during turning at 2-day intervals for 8 days (C1-C4). The numbers of streptococci, coagulase-negative staphylococci and coliforms were 2.6 × 10 , 1.7 × 10 and 1.0 × 10 colony-forming units (cfu)/g in CMS (C4) (summer), and these counts were markedly lower (P < 0.05) than those in CMS (C0 and C1). The bacterial counts ranged from 10 to 1.7 × 10 cfu/g in CMS (C4) (summer) and were within approved levels, <1 × 10 cfu/g, indicating a minimal mastitis risk. The temperatures in CMS (C1-C4) increased to 63°C-74°C in summer and 67°C-70°C in winter. The mean pH values in CMS (C0-C4) were 9.2 in summer and 8.7 in winter, and water contents ranged from 61.7% to 69.6% in summer and 73.2% to 66.2% in winter. The significant decrease of pathogenic bacteria in CMS appears to be closely related to temperature >63°C for 8 days, pH 8.7-9.2, and water content 62% to 73%. This study demonstrates that prepared CMS has value as a recycled material with the potential to alleviate udder health issues in dairy cows.
对由消化后的粪浆制备的堆肥固体（CMS）中引起乳腺炎的病原体、pH值和水分含量的变化进行了评估，在8天内每隔2天进行一次翻堆（C1 - C4）。夏季CMS（C4）中链球菌、凝固酶阴性葡萄球菌和大肠菌群的数量分别为2.6×10、1.7×10和1.0×10菌落形成单位（cfu）/克，这些计数显著低于（P < 0.05）CMS（C0和C1）中的计数。夏季CMS（C4）中的细菌计数范围为10至1.7×10 cfu/克，处于批准水平<1×10 cfu/克以内，表明乳腺炎风险极小。CMS（C1 - C4）中的温度在夏季升至63°C - 74°C，冬季升至67°C - 70°C。CMS（C0 - C4）的平均pH值夏季为9.2，冬季为8.7，夏季水分含量范围为61.7%至69.6%，冬季为73.2%至66.2%。CMS中病原菌的显著减少似乎与8天内温度>63°C、pH值8.7 - 9.2以及水分含量62%至73%密切相关。本研究表明，制备的CMS作为一种可回收材料具有价值，有可能缓解奶牛的乳房健康问题。
