Physical Chemistry I, Department of Chemistry and Biology & Research Center of Micro and Nanochemistry and Engineering (Cµ), University of Siegen, Adolf-Reichwein-Str. 2, 57076, Siegen, Germany.
Macromol Biosci. 2018 Feb;18(2). doi: 10.1002/mabi.201700317. Epub 2017 Dec 27.
Poly(di(ethylene glycol) methyl ether methacrylate) (PDEGMA) brushes show interesting thermoresponsive behavior that can be applied for cell release surfaces. Here it is shown that PDEGMA thickness gradients, which are synthesized by surface-initiated atom transfer radical polymerization, allow the systematic and precise analysis of the attachment of PaTu 8988 cells. By pumping the polymerization solution into the reactor with vertically fixed initiator samples, PDEGMA gradients with linearly increasing dry ellipsometric thickness with typical slopes of 2.5 nm cm are obtained. A very narrow transition of PaTu 8988t cell attachment is observed that starts for a thickness larger than 7.1 ± 0.2 nm. For PDEGMA layers thicker than 8.7 ± 0.2 nm no attached cells are found. This very narrow transition in brush properties within a thickness difference of <2 nm from cell-adherent to cell-nonadherent can be determined in much greater detail than before owing to the thickness gradients with shallow slope.
聚（二乙二醇）甲基醚甲基丙烯酸酯（PDEGMA）刷具有有趣的温敏行为，可应用于细胞释放表面。本文展示了通过表面引发原子转移自由基聚合合成的 PDEGMA 厚度梯度，可以对 PaTu 8988 细胞的附着进行系统和精确的分析。通过将聚合溶液泵入具有垂直固定引发剂样品的反应器中，可以获得具有典型斜率为 2.5nm/cm 的线性增加干椭圆度厚度的 PDEGMA 梯度。观察到 PaTu 8988t 细胞附着的非常窄的过渡，其起始厚度大于 7.1±0.2nm。对于厚度大于 8.7±0.2nm 的 PDEGMA 层，没有附着的细胞。由于具有浅斜率的厚度梯度，可以比以前更详细地确定在从细胞附着到非细胞附着的厚度差<2nm 范围内刷性能的这种非常窄的转变。
