Zhou Qi-Zhao, Liu Cun-Dong, Yang Jian-Kun, Guo Wen-Bin, Zhou Jun-Hao, Bian Jun
Department of Urology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong 510630, China.
Zhonghua Nan Ke Xue. 2016 Nov;22(11):963-967.
To investigate the changes in the percentage of myeloid-derived suppressor cells (MDSCs) in the peripheral blood of prostate cancer (PCa) patients and explore the correlation of MDSCs and their subsets with the prognosis of PCa.
Using flow cytometry, we determined the percentage of MDSCs and the levels of Arg-1, iNOS and PD-L1 in the peripheral blood of 32 PCa patients and 25 healthy controls, detected the distribution of CD14+ Mo-MDSC and CD15+ PMN-MDSC subsets, and analyzed the correlation between the obtained parameters and the prognosis of PCa.
Compared with the healthy controls, the PCa patients showed significant increases in the percentage of MDSCs (P<0.01) and levels of Arg-1, iNOS and PD-L1 in the peripheral blood. Statistically significant differences were observed in the distribution of the CD14+ Mo-MDSC and CD15+ PMN-MDSC subsets between the two groups（60.4% vs 72.2%, 29.5% vs 18.8%） (P<0.05). The percentages of MDSCs and Mo-MDSCs were remarkably correlated with the total survival rate of the PCa patients (P=0.025 and 0.017).
The percentages of MDSCs and CD14+ Mo-MDSCs in the peripheral blood were correlated with the prognosis of PCa, which may provide a target or some evidence for the clinical treatment of PCa.
探讨前列腺癌（PCa）患者外周血中髓源性抑制细胞（MDSCs）百分比的变化，并探究MDSCs及其亚群与PCa预后的相关性。
采用流式细胞术，测定32例PCa患者和25例健康对照者外周血中MDSCs的百分比以及精氨酸酶-1（Arg-1）、诱导型一氧化氮合酶（iNOS）和程序性死亡受体配体1（PD-L1）的水平，检测CD14+单核细胞源性MDSC（Mo-MDSC）和CD15+多形核细胞源性MDSC（PMN-MDSC）亚群的分布，并分析所获参数与PCa预后的相关性。
与健康对照者相比，PCa患者外周血中MDSCs百分比以及Arg-1、iNOS和PD-L1水平显著升高（P<0.01）。两组间CD14+ Mo-MDSC和CD15+ PMN-MDSC亚群的分布存在统计学显著差异（60.4% 对72.2%，29.5% 对18.8%）（P<0.05）。MDSCs和Mo-MDSCs的百分比与PCa患者的总生存率显著相关（P=0.025和0.017）。
外周血中MDSCs和CD14+ Mo-MDSCs的百分比与PCa预后相关，这可能为PCa的临床治疗提供靶点或某些依据。
