Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai 200241, China.
Cell Rep. 2017 Dec 26;21(13):3957-3969. doi: 10.1016/j.celrep.2017.11.111.
Pre-implantation embryo development is an intricate and precisely regulated process orchestrated by maternally inherited proteins and newly synthesized proteins following zygotic genome activation. Although genomic and transcriptomic studies have enriched our understanding of the genetic programs underlying this process, the protein expression landscape remains unexplored. Using quantitative mass spectrometry, we identified nearly 5,000 proteins from 8,000 mouse embryos of each stage (zygote, 2-cell, 4-cell, 8-cell, morula, and blastocyst). We found that protein expression in zygotes, morulas, and blastocysts is distinct from 2- to 8-cell embryos. Analysis of protein phosphorylation identified critical kinases and signal transduction pathways. We highlight key factors and their important roles in embryo development. Combined analysis of transcriptomic and proteomic data reveals coordinated control of RNA degradation, transcription, and translation and identifies previously undefined exon-junction-derived peptides. Our study provides an invaluable resource for further mechanistic studies and suggests core factors regulating pre-implantation embryo development.
胚胎植入前的发育是一个复杂而精确调控的过程，由母源遗传的蛋白质和受精卵基因组激活后新合成的蛋白质共同调控。虽然基因组和转录组研究丰富了我们对这一过程中遗传程序的理解，但蛋白质表达图谱仍未被探索。通过定量质谱分析，我们从每个阶段（受精卵、2 细胞、4 细胞、8 细胞、桑椹胚和囊胚）的 8000 个小鼠胚胎中鉴定了近 5000 种蛋白质。我们发现受精卵、桑椹胚和囊胚的蛋白质表达与 2 到 8 细胞胚胎不同。蛋白质磷酸化分析鉴定了关键的激酶和信号转导途径。我们重点介绍了胚胎发育中的关键因素及其重要作用。转录组和蛋白质组数据的综合分析揭示了 RNA 降解、转录和翻译的协调控制，并确定了以前未定义的外显子连接衍生肽。我们的研究为进一步的机制研究提供了宝贵的资源，并提出了调节胚胎植入前发育的核心因素。
