Hamada Nanako, Mizuno Makoto, Tomita Hiroyuki, Iwamoto Ikuko, Hara Akira, Nagata Koh-Ichi
Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya, Kasugai, 480-0392, Japan.
Department of Tumor Pathology, Gifu University Graduate School of Medicine, Gifu, 501-1194, Japan.
Med Mol Morphol. 2018 Jun;51(2):111-117. doi: 10.1007/s00795-017-0178-3. Epub 2017 Dec 27.
Dusp22 (dual-specificity phosphatase 22) is considered to regulate various cellular processes through the regulation of protein dephosphorylation. In this study, we prepared a specific antibody against Dusp22, anti-Dusp22, and carried out expression analyses with mouse tissues and cultured cell lines. Western blotting analyses demonstrated a tissue-dependent expression profile of Dusp22 in the adult mouse, and strongly suggested the presence of isoforms with larger molecular masses. In fibroblast NIH3T3 cells, while both endogenous and Myc-tagged Dusp22 was diffusely distributed in the cytoplasm, Myc-Dusp22 was partially colocalized with actin cytoskeleton. From the obtained results, anti-Dusp22 was found to be a useful tool for biochemical and cell biological analyses of Dusp22.
双特异性磷酸酶22（Dusp22）被认为通过调节蛋白质去磷酸化来调控各种细胞过程。在本研究中，我们制备了一种针对Dusp22的特异性抗体，即抗Dusp22抗体，并对小鼠组织和培养细胞系进行了表达分析。蛋白质免疫印迹分析表明，Dusp22在成年小鼠中呈现组织依赖性表达谱，并强烈提示存在分子量更大的异构体。在成纤维细胞NIH3T3细胞中，内源性和Myc标签的Dusp22均在细胞质中呈弥散分布，而Myc-Dusp22与肌动蛋白细胞骨架部分共定位。根据所得结果，发现抗Dusp22抗体是对Dusp22进行生化和细胞生物学分析的有用工具。
