Yu Wen, Xu Zhi-Peng, Che Xiao-Yan, Chen Yun, Dai Yu-Tian
Department of Andrology, Drum Tower Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210008, China.
Zhonghua Nan Ke Xue. 2016 Dec;22(12):1077-1082.
To explore aging-related changes in erectile function and the expressions of SIRT1 and other relevant factors in rats.
We divided 40 male SD rats into four age groups of equal number: 2-month-old (2 mo), 8-month-old (8 mo), 14-month-old (14 mo), and 20-month-old (20 mo), measured the intracavernous pressure (ICP), mean arterial pressure (MAP), and ICP/MAP ratio by electrostimulation of the cavernous nerve, evaluated fibrosis in the corpus cavernosum by Masson's trichrome staining, detected the expressions of SIRT1, P53, and FOXO3a by Western blot, and determined the levels of NO and cGMP using the NO/cGMP kit.
Both the ICP/MAP ratio and the cGMP level were elevated with aging, reaching the peak at 8 months and then gradually decreased. Masson staining showed an aging-related increase of collagen fibers in the corpus cavernosum.The expression of SIRT1 was reduced while those of P53 and FOXO3a increased with aging.
Aging-related erectile dysfunction may be attributed to the reduced activity of the NO/cGMP pathway, apoptosis and oxidative stress, and SIRT1 may play a role in aging-related erectile dysfunction.
探讨大鼠勃起功能与衰老相关的变化以及沉默信息调节因子1（SIRT1）和其他相关因子的表达情况。
将40只雄性SD大鼠平均分为4个年龄组：2月龄（2 mo）、8月龄（8 mo）、14月龄（14 mo）和20月龄（20 mo），通过电刺激海绵体神经测量海绵体内压（ICP）、平均动脉压（MAP）及ICP/MAP比值，采用Masson三色染色法评估海绵体纤维化情况，通过蛋白质免疫印迹法检测SIRT1、P53和叉头框蛋白O3a（FOXO3a）的表达，使用NO/cGMP试剂盒测定NO和cGMP水平。
随着衰老，ICP/MAP比值和cGMP水平均升高，在8个月时达到峰值，随后逐渐下降。Masson染色显示海绵体内胶原纤维随衰老增加。随着衰老，SIRT1表达降低，而P53和FOXO3a表达增加。
衰老相关的勃起功能障碍可能归因于NO/cGMP途径活性降低、细胞凋亡和氧化应激，且SIRT1可能在衰老相关的勃起功能障碍中发挥作用。
