Device Engineering, Nestlé Institute of Health Sciences, EPFL Innovation Park, Lausanne, CH-1015, Switzerland.
Mitochondrial Function, Nestlé Institute of Health Sciences, EPFL Innovation Park, Lausanne, CH-1015, Switzerland.
Adv Healthc Mater. 2018 Apr;7(8):e1701148. doi: 10.1002/adhm.201701148. Epub 2017 Dec 28.
Managing increasingly prevalent chronic diseases will require close continuous monitoring of patients. Cell-based biosensors may be used for implantable diagnostic systems to monitor health status. Cells are indeed natural sensors in the body. Functional cellular systems can be maintained in the body for long-term implantation using cell encapsulation technology. By taking advantage of recent progress in miniaturized optoelectronic systems, the genetic engineering of optically responsive cells may be combined with cell encapsulation to generate smart implantable cell-based sensing systems. In biomedical research, cell-based biosensors may be used to study cell signaling, therapeutic effects, and dosing of bioactive molecules in preclinical models. Today, a wide variety of genetically encoded fluorescent sensors have been developed for real-time imaging of living cells. Here, recent developments in genetically encoded sensors, cell encapsulation, and ultrasmall optical systems are highlighted. The integration of these components in a new generation of biosensors is creating innovative smart in vivo cell-based systems, bringing novel perspectives for biomedical research and ultimately allowing unique health monitoring applications.
管理日益流行的慢性疾病将需要对患者进行密切的持续监测。基于细胞的生物传感器可用于植入式诊断系统来监测健康状况。细胞实际上是体内的天然传感器。通过细胞封装技术，可以维持功能性细胞系统以进行长期植入。利用最近在小型化光电系统方面的进展，可以将对光有响应的细胞的基因工程与细胞封装相结合，从而生成智能可植入的基于细胞的传感系统。在生物医学研究中，基于细胞的生物传感器可用于研究细胞信号、治疗效果以及临床前模型中生物活性分子的剂量。如今，已经开发出了多种用于实时成像活细胞的基因编码荧光传感器。本文重点介绍了基因编码传感器、细胞封装和超小型光学系统方面的最新进展。这些组件在新一代生物传感器中的集成正在创建创新的智能体内基于细胞的系统，为生物医学研究带来新的视角，并最终允许进行独特的健康监测应用。
