Galectin 3 在原发性口腔鳞状细胞癌中的表达。
Galectin 3 expression in primary oral squamous cell carcinomas.
机构信息
Department of Oral and Maxillofacial Surgery, Friedrich-Alexander University Erlangen-Nürnberg, Glueckstrasse 11, 91054, Erlangen, Germany.
Institute of Pathology, Department of Nephropathology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany.
出版信息
BMC Cancer. 2017 Dec 29;17(1):906. doi: 10.1186/s12885-017-3920-2.
BACKGROUND
Immunologic factors can promote the progression of oral squamous cell carcinomas (oscc). The phylogenetic highly conserved protein Galectin 3 (Gal3) contributes to cell differentiation and immune homeostasis. There is evidence that Gal3 is involved in the progression of oscc and influences the regulation of macrophage polarization. Macrophage polarization (M1 vs. M2) in solid malignancies like oscc contributes to tumor immune-escape. However, the relationship between macrophage polarization and Gal3 expression in oscc is not yet understood. The current study analyzes the association between histomorphologic parameters (T-, N-, L- Pn-status, grading) and Gal3 expression resp. the ratio between Gal3 expressing cells and CD68 positive macrophages in oscc specimens.
METHODS
Preoperative diagnostic biopsies (n = 26) and tumor resection specimens (n = 34) of T1/T2 oscc patients were immunohistochemically analyzed for Gal3 and CD68 expression. The number of Gal3 expressing cells and the ratio between CD68 and Gal3 expressing cells was quantitatively assessed.
RESULTS
In biopsy and tumor resection specimens, the number of Gal3 positive cells as well as the Gal3/CD68 ratio were significantly (p < 0.05) higher in T2 oscc compared to T1 cases. In biopsy specimens, a significantly (p < 0.05) increased Gal3 expression and Gal3/CD68 ratio was associated with the progression marker lymph vessel infiltration (L1). Tumor resection specimens of cases with lymph node metastases (N+) had a significantly (p < 0.05) increased Gal3 expression. Additionally, a high Gal3/CD68 ratio correlated significantly (p < 0.05) with higher grading (G3) in tumor resection specimens.
CONCLUSION
High Gal3 expression in oscc is associated with tumor size (T-status) and parameters of malignancy (N-, L-status, grading). Gal3 might contribute to M2 macrophage mediated local immune tolerance. Gal3 expression shows association with prognosis in oscc and represent a potential therapeutic target.
背景
免疫因素可促进口腔鳞状细胞癌（oscc）的进展。系统发育高度保守的蛋白半乳糖凝集素 3（Gal3）有助于细胞分化和免疫稳态。有证据表明，Gal3 参与 oscc 的进展，并影响巨噬细胞极化的调节。在像 oscc 这样的实体恶性肿瘤中，巨噬细胞极化（M1 与 M2）有助于肿瘤免疫逃避。然而，巨噬细胞极化与 oscc 中 Gal3 表达之间的关系尚不清楚。本研究分析了组织形态学参数（T、N、L-Pn 状态、分级）与 Gal3 表达之间的关系，以及 Gal3 表达细胞与 oscc 标本中 CD68 阳性巨噬细胞之间的比值。
方法
对 T1/T2 oscc 患者的术前诊断性活检（n=26）和肿瘤切除标本（n=34）进行免疫组织化学分析，以检测 Gal3 和 CD68 的表达。定量评估 Gal3 表达细胞的数量以及 CD68 和 Gal3 表达细胞之间的比值。
结果
在活检和肿瘤切除标本中，T2 oscc 中 Gal3 阳性细胞的数量以及 Gal3/CD68 比值明显高于 T1 病例（p<0.05）。在活检标本中，Gal3 表达和 Gal3/CD68 比值的增加与淋巴管浸润（L1）的进展标志物显著相关（p<0.05）。有淋巴结转移（N+）的肿瘤切除标本中 Gal3 表达明显增加（p<0.05）。此外，肿瘤切除标本中高 Gal3/CD68 比值与分级（G3）显著相关（p<0.05）。
结论
oscc 中 Gal3 的高表达与肿瘤大小（T 分期）和恶性程度参数（N、L 状态、分级）相关。Gal3 可能有助于 M2 巨噬细胞介导的局部免疫耐受。Gal3 表达与 oscc 的预后相关，是一个潜在的治疗靶点。
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