Prasad S C, Boyle J, Dritschilo A
Department of Radiation Medicine, Vincent T. Lombardi Comprehensive Cancer Research Center, Georgetown University Medical Center, Washington, D.C. 20007.
Radiat Res. 1989 Mar;117(3):538-46.
A sensitive quantitation of DNA (0.2 to 10 ng) can be achieved using a 32P-labeled Alu probe to hybridize human DNA spotted onto nylon membrane. This allows the determination of radiation-induced single-strand breaks without the use of [3H]thymidine prelabeling of cells in culture. The sensitivity of this technique in HeLa cells is comparable to results obtained using the alkaline unwinding technique. The method is applicable to cells in both exponential and plateau phases of growth.
使用32P标记的Alu探针与点在尼龙膜上的人类DNA杂交，可实现对0.2至10纳克DNA的灵敏定量。这使得在不使用[3H]胸腺嘧啶核苷对培养细胞进行预标记的情况下，就能测定辐射诱导的单链断裂。该技术在HeLa细胞中的灵敏度与使用碱性解旋技术获得的结果相当。此方法适用于处于指数生长期和平稳期的细胞。
