[大鼠照射胸腺细胞中DNA核小体间断裂的电泳分析]
[Electrophoretic analysis of the internucleosome fragmentation of DNA in irradiated thymocytes of rats].
作者信息
Riabchenko N I, Ivannik B P, Sin'kova R V, Konov A V
出版信息
Radiobiologiia. 1989 Jan-Feb;29(1):48-53.
PMID:2928484
Abstract
Total DNA and DNA of chromatin degradation products obtained from rat thymocytes 6 h after irradiation with a dose of 10 Gy were separated electrophoretically. Relative shares of mononucleosomes and their oligomers were determined. Experimental distributions of DNA fragments differ from those calculated on the basis of the assumption of a random breakage of bonds between the nucleosomes.
摘要
对受10 Gy剂量照射6小时后的大鼠胸腺细胞所获得的总DNA和染色质降解产物的DNA进行了电泳分离。测定了单核小体及其寡聚体的相对比例。DNA片段的实验分布与基于核小体间键随机断裂假设所计算出的分布不同。
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