Yang Qian, Li Xiao-Peng, Zhong Yuan-Bin, Xiang Tian-Xin, Zhang Lun-Li
Department of Infectious Disease, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
Exp Ther Med. 2017 Dec;14(6):5924-5930. doi: 10.3892/etm.2017.5288. Epub 2017 Oct 11.
Hepatitis B virus (HBV) X protein (HBx) serves an important role in HBV infection and the development of HBV-related liver cancer. Interferon-α (IFN-α) is used to treat patients with HBV; however, the role of IFN-α in the development of HBV-related liver cancer remains unclear. The present study established a new HBV-related liver cancer model (Huh-7-HBx) by transfecting the hepatoma cell line Huh-7, with HBx-expressing lentivirus. Following IFN-α treatment, cell viability, migration and invasion, as well as the expression of antiviral proteins in Huh-7-HBx, were subsequently determined. The results demonstrated that HBx-expressing lentivirus had no significant effect on cell viability but promoted the migration and invasion of Huh-7 cells. The expression of the antiviral genes IFN α and β receptor subunit 1 (IFNAR1), IFNAR2, IFN-stimulated gene factor 3, double-stranded RNA-activated protein kinase and ribonuclease L, was also increased. Following treatment of Huh-7-HBx cells with IFN-α, the expression of antiviral genes was increased at the level of transcription and translation, whereas cell migration and invasion was decreased. The present study suggests that IFN-α may attenuate the development of HBV-related liver cancer by reducing cell migration and invasion and promoting the expression of antiviral proteins.
乙型肝炎病毒（HBV）X蛋白（HBx）在HBV感染及HBV相关肝癌的发生发展中发挥重要作用。干扰素-α（IFN-α）用于治疗HBV患者；然而，IFN-α在HBV相关肝癌发生发展中的作用仍不清楚。本研究通过用表达HBx的慢病毒转染肝癌细胞系Huh-7，建立了一种新的HBV相关肝癌模型（Huh-7-HBx）。在IFN-α处理后，随后测定了Huh-7-HBx细胞的活力、迁移和侵袭能力，以及抗病毒蛋白的表达。结果表明，表达HBx的慢病毒对细胞活力无显著影响，但促进了Huh-7细胞的迁移和侵袭。抗病毒基因IFNα和β受体亚基1（IFNAR1）、IFNAR2、IFN刺激基因因子3、双链RNA激活蛋白激酶和核糖核酸酶L的表达也增加。用IFN-α处理Huh-7-HBx细胞后，抗病毒基因的表达在转录和翻译水平均增加，而细胞迁移和侵袭能力则下降。本研究表明，IFN-α可能通过减少细胞迁移和侵袭并促进抗病毒蛋白的表达来减轻HBV相关肝癌的发展。
