Chua Jennifer, Fisher Nathan A, Falcinelli Shane D, DeShazer David, Friedlander Arthur M
Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases;
Southern Research.
J Vis Exp. 2017 Nov 24(129):56491. doi: 10.3791/56491.
Many aspects of innate immunity are conserved between mammals and insects. An insect, the Madagascar hissing cockroach from the genus Gromphadorhina, can be utilized as an alternative animal model for the study of virulence, host-pathogen interaction, innate immune response, and drug efficacy. Details for the rearing, care and breeding of the hissing cockroach are provided. We also illustrate how it can be infected with bacteria such as the intracellular pathogens Burkholderia mallei, B. pseudomallei, and B. thailandensis. Use of the hissing cockroach is inexpensive and overcomes regulatory issues dealing with the use of mammals in research. In addition, results found using the hissing cockroach model are reproducible and similar to those obtained using mammalian models. Thus, the Madagascar hissing cockroach represents an attractive surrogate host that should be explored when conducting animal studies.
哺乳动物和昆虫在先天免疫的许多方面是保守的。一种昆虫，即来自Gromphadorhina属的马达加斯加发声蟑螂，可作为研究毒力、宿主-病原体相互作用、先天免疫反应和药物疗效的替代动物模型。本文提供了发声蟑螂的饲养、照料和繁殖细节。我们还说明了如何用细胞内病原体如鼻疽伯克霍尔德菌、类鼻疽伯克霍尔德菌和泰国伯克霍尔德菌等细菌感染它。使用发声蟑螂成本低廉，并且克服了在研究中使用哺乳动物所涉及的监管问题。此外，使用发声蟑螂模型获得的结果具有可重复性，并且与使用哺乳动物模型获得的结果相似。因此，马达加斯加发声蟑螂是一种有吸引力的替代宿主，在进行动物研究时值得探索。
