次致死浓度的羟基脲增强了间充质干细胞在大鼠脊髓损伤模型中的保护作用。
Sublethal concentration of HO enhances the protective effect of mesenchymal stem cells in rat model of spinal cord injury.
作者信息
Rahimi Asrin, Amiri Iraj, Roushandeh Amaneh Mohammadi, Choshali Zoleikha Golipour, Alizadeh Zohreh, Artimani Tayebeh, Afshar Saeid, Asl Sara Soleimani
机构信息
Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Anatomy Department, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
出版信息
Biotechnol Lett. 2018 Mar;40(3):609-615. doi: 10.1007/s10529-017-2499-7. Epub 2017 Dec 29.
OBJECTIVE
To investigate the effect of HO on the migration and antioxidant defense of mesenchymal stem cells (MSCs) and the neurotrophic effects of HO-treated MSCs on spinal cord injury (SCI).
RESULTS
Sublethal concentrations of HO decreased cell migration and expression of CXCR4 and CCR2 as well as Nrf2 expression in MSCs. In the second phase, transplantation of treated and untreated MSCs to SCI caused minor changes in locomotor dysfunction. There was a significantly difference between cell-treated and spinal cord injury groups in expression of BDNF (brain-derived neurotrophic factor). Transplantation of HO-treated cells caused an increase in BDNF expression compared to non-treated cells.
CONCLUSION
Transplantation of HO-treated stem cells may have protective effects against SCI through by increasing neurotrophic factors.
目的
探讨羟基脲（HO）对间充质干细胞（MSCs）迁移和抗氧化防御的影响，以及经HO处理的MSCs对脊髓损伤（SCI）的神经营养作用。
结果
亚致死浓度的HO降低了MSCs的细胞迁移、CXCR4和CCR2的表达以及Nrf2的表达。在第二阶段，将处理过和未处理过的MSCs移植到SCI模型中，对运动功能障碍的影响较小。细胞处理组和脊髓损伤组在脑源性神经营养因子（BDNF）表达上存在显著差异。与未处理的细胞相比，经HO处理的细胞移植后BDNF表达增加。
结论
经HO处理的干细胞移植可能通过增加神经营养因子对SCI具有保护作用。
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