Roh Gwang Hyun, Park Kye Chung, Oh Hyun-Woo, Park Chung Gyoo
Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea.
New Zealand Institute for Plant and Food Research, Christchurch, New Zealand.
Micron. 2018 Mar;106:7-20. doi: 10.1016/j.micron.2017.12.006. Epub 2017 Dec 21.
We investigated the morphology and distribution of antennal sensilla in males and females of two tortricid moths, Epiphyas postvittana and Planotortrix octo, by scanning electron microscopy. The number and overall length of flagellomeres were significantly greater in females than in males in both species. The antennae of each species bearing six morphological types of sensilla (trichodea, basiconica, coeloconica, auricillica, chaetica, and styloconica), with different numbers and distributions along the antennae. Among these sensilla, four types (trichodea, basiconica, coeloconica, and auricillica) displayed multi-porous cuticular surfaces, indicating that their primary sensory function is olfactory. Each of these four types of sensilla could be further classified into subtypes according to their size, shape, and surface structure. Both E. postvittana and P. octo exhibited sexual dimorphism of the profiles of antennal olfactory sensilla. Trichoid sensilla were the most abundant type in both species. Subtype I trichoid sensilla were male-specific in both species, indicating that they are responsible for the perception of conspecific female sex pheromone. By contrast, subtype II trichoid sensilla were more abundant in female antennae in both species, suggesting that some subtype II trichoid sensilla are involved in female-specific behaviors, such as oviposition. Chaetic and styloconic sensilla displayed relatively even distributions along the antennae. Our results indicate that the antennae of E. postvittana and P. octo have species-specific and sex-specific profiles of olfactory sensilla. The morphological information obtained in our study provides a basis for electrophysiological and behavioral studies of the olfactory sensory function of each morphological type of sensilla.
我们通过扫描电子显微镜研究了两种卷蛾，即苹果小卷蛾（Epiphyas postvittana）和八点小卷蛾（Planotortrix octo）雌雄成虫触角感器的形态和分布。在这两个物种中，雌虫触角鞭节的数量和总长度均显著多于雄虫。每个物种的触角都有六种形态类型的感器（毛形感器、锥形感器、腔锥形感器、耳形感器、刺形感器和栓锥形感器），它们在触角上的数量和分布各不相同。在这些感器中，四种类型（毛形感器、锥形感器、腔锥形感器和耳形感器）具有多孔的表皮表面，表明它们的主要感觉功能是嗅觉。这四种类型的感器中的每一种都可以根据其大小、形状和表面结构进一步细分为亚型。苹果小卷蛾和八点小卷蛾在触角嗅觉感器的形态上均表现出两性差异。毛形感器是这两个物种中最丰富的类型。I型毛形感器在两个物种中均为雄性特有，表明它们负责感知同种雌性性信息素。相比之下，II型毛形感器在两个物种的雌虫触角中更为丰富，这表明一些II型毛形感器参与了雌虫特有的行为，如产卵。刺形感器和栓锥形感器在触角上的分布相对均匀。我们的结果表明，苹果小卷蛾和八点小卷蛾的触角具有物种特异性和性别特异性的嗅觉感器形态。我们研究中获得的形态学信息为每种形态类型感器的嗅觉感觉功能的电生理学和行为学研究提供了基础。
