Hyslop Jesse S, McGettrick Julie R, Hall Leah M G, Chuk Hungngai, Palmer Christopher P
Department of Chemistry and Biochemistry, University of Montana, Missoula, MT, USA.
Department of Chemistry and Biochemistry, University of Montana, Missoula, MT, USA.
Anal Chim Acta. 2018 Feb 13;1000:293-302. doi: 10.1016/j.aca.2017.11.020. Epub 2017 Nov 14.
The fundamental relationships between the structure and chemistry of latex nanoparticles synthesized by reversible addition fragmentation chain transfer (RAFT) controlled living polymerization and their subsequent performance as pseudostationary phases (PSP) are reported in this paper. RAFT enables the rational optimization of latex nanoparticle pseudostationary phases and control of the behavior of the PSP. Nanoparticles comprised of amphiphilic diblock copolymers of 2-acrylamido-2-methylpropane sulfonic acid-derived ionic/hydrophilic blocks and butyl- ethyl- or methyl-acrylate-derived hydrophobic blocks were synthesized in two sizes. The mobility, methylene selectivity, and efficiency of each of the six pseudostationary phases are reported, as well as the relationship between monomer quantity and NP size. Linear solvation energy relationships are reported and compared to SDS micelles and previous nanoparticle pseudostationary phases. The solvation characteristics and selectivity of nanoparticle pseudostationary phases is shown to be affected primarily by the structure of the hydrophobic copolymer block. Butyl acrylate nanoparticles 17 nm in diameter are found to provide the best overall separation performance with over 500 thousand theoretical plates generated in 6 min separations.
本文报道了通过可逆加成-断裂链转移（RAFT）可控活性聚合合成的胶乳纳米颗粒的结构与化学性质之间的基本关系，以及它们作为准固定相（PSP）的后续性能。RAFT能够合理优化胶乳纳米颗粒准固定相并控制PSP的行为。由2-丙烯酰胺基-2-甲基丙烷磺酸衍生的离子/亲水嵌段与丙烯酸丁酯、丙烯酸乙酯或丙烯酸甲酯衍生的疏水嵌段组成的两亲性二嵌段共聚物纳米颗粒被合成了两种尺寸。报告了六个准固定相各自的迁移率、亚甲基选择性和效率，以及单体数量与纳米颗粒尺寸之间的关系。报告了线性溶剂化能关系，并与十二烷基硫酸钠胶束和先前的纳米颗粒准固定相进行了比较。结果表明，纳米颗粒准固定相的溶剂化特性和选择性主要受疏水共聚物嵌段结构的影响。发现直径为17 nm的丙烯酸丁酯纳米颗粒在6分钟的分离中能产生超过50万块理论塔板，提供了最佳的整体分离性能。
