Department of Thoracic Surgery, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, Henan China; Department of Thoracic Surgery, The First Affiliated Hospital of Henan University, Kaifeng 475000, Henan China.
Department of Thoracic Surgery, The First Affiliated Hospital of Henan University, Kaifeng 475000, Henan China.
Biomed Pharmacother. 2018 Feb;98:594-599. doi: 10.1016/j.biopha.2017.12.080. Epub 2017 Dec 28.
Long non-coding RNA HNF1A-antisense 1 (lncRNA HNF1A-AS1) plays important roles in the progression of human tumors. The aim of this study is to unravel the underlying mechanism of HNF1A-AS1 in non-small cell lung cancer (NSCLC). In the present study, we found that HNF1A-AS1 was upregulation in NSCLC tissues and cell lines. High HNF1A-AS1 expression was associated with patients' advanced TNM stage and lymph node metastasis. Reduced HNF1A-AS1 expression inhibited lung cancer cells proliferation, invasion and increased cells apoptosis rate. Bioinformatics analysis and luciferase reporter assay revealed that HNF1A-AS1 interacted with miR-17-5p by directly targeting it. Rescue experiments showed that miR-17-5p suppression reversed the tumor-suppressing role of HNF1A-AS1 knockdown on NSCLC progression. Conclusion, our data indicated that lncRNA HNF1A-AS1 promoted lung cancer cells proliferation and invasion via regulating miR-17-5p, suggesting that HNF1A-AS1 could act as a potent therapeutic strategy for the treatment of NSCLC patients.
长链非编码 RNA HNF1A-反义 1（lncRNA HNF1A-AS1）在人类肿瘤的进展中发挥重要作用。本研究旨在揭示 HNF1A-AS1 在非小细胞肺癌（NSCLC）中的潜在机制。在本研究中，我们发现 HNF1A-AS1 在 NSCLC 组织和细胞系中上调。高 HNF1A-AS1 表达与患者的晚期 TNM 分期和淋巴结转移有关。降低 HNF1A-AS1 表达抑制肺癌细胞增殖、侵袭并增加细胞凋亡率。生物信息学分析和荧光素酶报告基因实验表明，HNF1A-AS1 通过直接靶向 miR-17-5p 与其相互作用。挽救实验表明，miR-17-5p 抑制逆转了 HNF1A-AS1 敲低对 NSCLC 进展的肿瘤抑制作用。结论表明，lncRNA HNF1A-AS1 通过调节 miR-17-5p 促进肺癌细胞增殖和侵袭，提示 HNF1A-AS1 可作为治疗 NSCLC 患者的有效治疗策略。
