Di Giulio Andrea, Muzzi Maurizio
Department of Science, University Roma Tre, Viale G. Marconi, 446, 00146 Rome, Italy; Laboratorio Interdipartimentale di Microscopia Elettronica (LIME), University Roma Tre, Rome, Italy.
Department of Science, University Roma Tre, Viale G. Marconi, 446, 00146 Rome, Italy; Laboratorio Interdipartimentale di Microscopia Elettronica (LIME), University Roma Tre, Rome, Italy.
Micron. 2018 Mar;106:21-26. doi: 10.1016/j.micron.2017.12.007. Epub 2017 Dec 21.
Transmission Electron Microscopy (TEM) has always been the conventional method to study arthropod ultrastructure, while the use of Scanning Electron Microscopy (SEM) was mainly devoted to the examination of the external cuticular structures by secondary electrons. The new generation field emission SEMs are capable to generate images at sub-cellular level, comparable to TEM images employing backscattered electrons. The potential of this kind of acquisition becomes very powerful in the dual beam FIB/SEM where the SEM column is combined with a Focused Ion Beam (FIB) column. FIB uses ions as a nano-scalpel to slice samples fixed and embedded in resin, replacing traditional ultramicrotomy. We here present two novel methods, which optimize the use of FIB/SEM for studying arthropod anatomy.
透射电子显微镜（TEM）一直是研究节肢动物超微结构的传统方法，而扫描电子显微镜（SEM）的应用主要集中于通过二次电子来检查外部表皮结构。新一代场发射扫描电子显微镜能够在亚细胞水平生成图像，可与使用背散射电子的透射电子显微镜图像相媲美。这种采集方式的潜力在双束聚焦离子束/扫描电子显微镜（FIB/SEM）中变得非常强大，其中扫描电子显微镜柱与聚焦离子束（FIB）柱相结合。聚焦离子束使用离子作为纳米手术刀来切割固定并包埋在树脂中的样本，取代了传统的超薄切片术。我们在此介绍两种新颖的方法，它们优化了FIB/SEM在研究节肢动物解剖学方面的应用。
