分离的大鼠胰岛中胰岛素原合成的调节

Regulation of proinsulin synthesis in isolated rat islets.

作者信息

Baird N M, Bauer G E

出版信息

Proc Soc Exp Biol Med. 1976 Jan;151(1):16-21. doi: 10.3181/00379727-151-39134.

Abstract

(1) A system is described for studying the short-term effects of agents on proinsulin synthesis in vitro, as measured by the incorporation of [3H]leucine into isolated proinsulin. (2) Of the agents tested, glucose has the most marked, and apparently earliest, effect on proinsulin synthesis. (3) The adenyl cyclase system participates in the regulation of proinsulin synthesis since exogenous cyclic AMP, glucagon, and caffeine are stimulatory. When cyclic AMP is added to the medium in the presence of glucose, it is the most potent agent acting on the adenyl cyclase-phosphodiesterase system. (4) The addition of NADPH to isolated rat islets inhibits proinsulin and Bulk Protein synthesis in vitro.

摘要

(1) 描述了一种用于研究试剂对体外胰岛素原合成的短期影响的系统，该影响通过将[3H]亮氨酸掺入分离的胰岛素原中进行测定。(2) 在测试的试剂中，葡萄糖对胰岛素原合成具有最显著且显然是最早的影响。(3) 腺苷酸环化酶系统参与胰岛素原合成的调节，因为外源性环磷酸腺苷、胰高血糖素和咖啡因具有刺激作用。当在葡萄糖存在的情况下将环磷酸腺苷添加到培养基中时，它是作用于腺苷酸环化酶 - 磷酸二酯酶系统的最有效试剂。(4) 向分离的大鼠胰岛中添加烟酰胺腺嘌呤二核苷酸磷酸（NADPH）会在体外抑制胰岛素原和总蛋白的合成。