Birkegård Anna Camilla, Halasa Tariq, Folkesson Anders, Clasen Julie, Græsbøll Kaare, Toft Nils
National Veterinary Institute, Technical University of Denmark, Kemitorvet, Building 204, 2800 Kgs. Lyngby, Denmark.
National Veterinary Institute, Technical University of Denmark, Kemitorvet, Building 204, 2800 Kgs. Lyngby, Denmark.
Prev Vet Med. 2018 Jan 1;149:10-14. doi: 10.1016/j.prevetmed.2017.11.007. Epub 2017 Nov 6.
Antimicrobial resistance in pigs has been under scrutiny for many years. However, many questions remain unanswered, including whether the initial antimicrobial resistance level of a pig will influence the antimicrobial resistance found at slaughter. Faecal samples from finishers pigs from 681 farms and from sows from 82 farms were collected, and levels of seven antimicrobial resistance genes, ermB, ermF, sulI, sulII, tet(M), tet(O), and tet(W), were quantified by high-capacity qPCR. There were 40 pairs of observations where the finishers were born in the farms of the sows. The objective of this study was to evaluate whether the levels of AMR genes found in finisher pigs at slaughter were associated with the levels in the farm where the finishers were born, and whether the levels of the AMR genes were equal in the sow and finisher pig populations. We found a significant positive correlation between the levels of AMR genes in finishers and the sows in the farms where the pigs were born for some of the genes (ermB (ρ=0.47, p-value=0.002), ermF (ρ=0.41, p-value=0.03), and tet(O) (ρ=0.33, p-value=0.04)). Furthermore, there were significant differences between AMR gene levels for the sow and finisher populations for ermB, ermF, sulI, sulII, tet(O), and tet(W), though not for tet(M).
猪的抗菌药物耐药性问题已受到多年关注。然而，许多问题仍未得到解答，包括猪的初始抗菌药物耐药水平是否会影响屠宰时检测到的抗菌药物耐药性。采集了来自681个农场的育肥猪和82个农场的母猪的粪便样本，并通过高容量定量PCR对ermB、ermF、sulI、sulII、tet(M)、tet(O)和tet(W)这7种抗菌药物耐药基因的水平进行了定量分析。有40对观察对象，其中育肥猪出生于母猪所在的农场。本研究的目的是评估屠宰时育肥猪中发现的抗菌药物耐药基因水平是否与其出生农场中的水平相关，以及母猪和育肥猪群体中抗菌药物耐药基因水平是否相等。我们发现，对于某些基因（ermB（ρ=0.47，p值=0.002）、ermF（ρ=0.41，p值=0.03）和tet(O)（ρ=0.33，p值=0.04）），育肥猪和出生农场中的母猪的抗菌药物耐药基因水平之间存在显著正相关。此外，ermB、ermF、sulI、sulII、tet(O)和tet(W)在母猪和育肥猪群体中的抗菌药物耐药基因水平存在显著差异，不过tet(M)没有差异。
