Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
Plants for Human Health Institute, Department of Animal Sciences, North Carolina State University, Kannapolis, NC, USA.
Oxid Med Cell Longev. 2017;2017:4256519. doi: 10.1155/2017/4256519. Epub 2017 Sep 24.
While surgery is the definitive treatment for early-stage melanoma, the current therapies against advanced melanoma do not yet provide an effective, long-lasting control of the lesions and a satisfactory impact on patient survival. Thus, research is also focused on novel treatments that could potentiate the current therapies. In the present study, we evaluated the effect of potassium ascorbate with ribose (PAR) treatment on the human melanoma cell line, A375, in 2D and 3D models. In the 2D model, in line with the current literature, the pharmacological treatment with PAR decreased cell proliferation and viability. In addition, an increase in Connexin 43 mRNA and protein was observed. This novel finding was confirmed in PAR-treated melanoma cells cultured in 3D, where an increase in functional gap junctions and a higher spheroid compactness were observed. Moreover, in the 3D model, a remarkable decrease in the size and volume of spheroids was observed, further supporting the treatment efficacy observed in the 2D model. In conclusion, our results suggest that PAR could be used as a safe adjuvant approach in support to conventional therapies for the treatment of melanoma.
虽然手术是早期黑色素瘤的明确治疗方法，但目前针对晚期黑色素瘤的治疗方法尚未提供对病变的有效、持久控制，也未能显著改善患者的生存情况。因此，研究也集中在可能增强现有治疗方法的新疗法上。在本研究中，我们评估了抗坏血酸钾与核糖（PAR）处理对人黑色素瘤细胞系 A375 的二维和三维模型的影响。在二维模型中，与现有文献一致，PAR 的药物治疗降低了细胞增殖和活力。此外，还观察到间隙连接蛋白 43 mRNA 和蛋白的增加。这一新发现得到了在 PAR 处理的三维培养的黑色素瘤细胞中的证实，其中观察到功能性缝隙连接增加和球体更加紧密。此外，在三维模型中，观察到球体的大小和体积明显减小，进一步支持了在二维模型中观察到的治疗效果。总之，我们的研究结果表明，PAR 可用作支持常规治疗黑色素瘤的安全辅助方法。
