Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, E-08193, Bellaterra, Barcelona, Spain.
Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, E-08193, Bellaterra, Barcelona, Spain.
Chem Phys Lipids. 2018 May;212:138-143. doi: 10.1016/j.chemphyslip.2017.12.006. Epub 2017 Dec 30.
Mycobacterium smegmatis is often used to study the different components of mycobacterial cell wall. Mycolic acids are important components of mycobacterial cell wall that have been associated with virulence. Recently, a novel lipid containing mycolic acids has been described in M. smegmatis. However, some uncertainties regarding the structure of this molecule named mycolate ester wax have been reported. The objective of this work was to perform an in depth structural study of this molecule for its precise characterization. Using H and C NMR spectroscopy, the molecular structure of mycolate ester wax found in M. smegmatis has been elucidated. The characterization was complemented with MS analyses. This molecule is formed by a carbon chain with three methyl substituted olefinic units and a mycolate structure with trans double bonds and cis cyclopropane rings. The present molecular study will facilitate the detection and identification of pentatriacontatrienyl mycolate in future studies by the performance of a simple 1D H NMR experiment.
耻垢分枝杆菌常被用于研究分枝杆菌细胞壁的不同组成部分。分枝菌酸是分枝杆菌细胞壁的重要组成部分，与毒力有关。最近，在耻垢分枝杆菌中描述了一种含有分枝菌酸的新型脂质。然而，关于这种名为脂酸酯蜡的分子的结构，存在一些不确定因素。这项工作的目的是对该分子进行深入的结构研究，以对其进行精确表征。利用 H 和 C NMR 光谱，阐明了在耻垢分枝杆菌中发现的脂酸酯蜡的分子结构。通过 MS 分析进行了补充。该分子由具有三个甲基取代的烯丙基单元的碳链和具有反式双键和顺式环丙烷环的脂酸结构组成。目前的分子研究将通过进行简单的 1D H NMR 实验，为未来的研究中检测和鉴定三十五碳烯脂酸提供便利。
