Wang Wei-Feng, Chen Peng, Lv Jing, Chen Lei, Sun Yu-He
Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China; Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China.
College of Plant Protection, China Agricultural University, Beijing 100193, China.
Gene. 2018 Mar 10;646:169-180. doi: 10.1016/j.gene.2017.12.053. Epub 2017 Dec 29.
Topping is an important agronomic practice that significantly impacts the yield of various crop plants. Topping and the regulation of axillary shoot outgrowth are common agronomic practices in tobacco. However, the effects of topping on gene expression in tobacco remain unknown. We applied the Illumina HiSeq™ 2000 platform and analyzed differentially expressed genes (DEGs) from untopped and topped plants to study the global changes in gene expression in response to topping. We found that the number of DEGs varied from 7609 to 18,770 based on the reads per kilobase per million mapped reads (RPKM) values. The Gene Ontology (GO) enrichment analysis revealed that the cellular carbohydrate metabolic process and the disaccharide metabolic process, which may contribute to starch accumulation and stress/defense, were overrepresented terms for the DEGs. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that many DEGs were involved in starch and sucrose metabolism, glycolysis/gluconeogenesis, pyruvate metabolism, and plant hormone signal transduction, among other processes. The knowledge gained will improve our understanding of the processes of axillary shoot formation and enlargement at the transcriptional level. This study lays a solid foundation for future studies on molecular mechanisms underlying the growth of axillary shoots.
打顶是一项重要的农艺措施，对多种作物的产量有显著影响。打顶以及对腋芽生长的调控是烟草种植中的常见农艺措施。然而，打顶对烟草基因表达的影响仍不清楚。我们应用Illumina HiSeq™ 2000平台，分析了未打顶和打顶植株的差异表达基因（DEG），以研究打顶后基因表达的整体变化。我们发现，基于每百万映射读数中每千碱基的读数（RPKM）值，差异表达基因的数量在7609至18770之间变化。基因本体论（GO）富集分析表明，可能有助于淀粉积累和应激/防御的细胞碳水化合物代谢过程和二糖代谢过程是差异表达基因的过度代表术语。京都基因与基因组百科全书（KEGG）分析表明，许多差异表达基因参与淀粉和蔗糖代谢、糖酵解/糖异生、丙酮酸代谢以及植物激素信号转导等过程。所获得的知识将提高我们在转录水平上对腋芽形成和生长过程的理解。本研究为未来关于腋芽生长分子机制的研究奠定了坚实基础。
