宫内暴露于脂多糖会改变犊牛出生后的代谢反应。
Exposure to lipopolysaccharide in utero alters the postnatal metabolic response in heifers.
出版信息
J Anim Sci. 2017 Dec;95(12):5176-5183. doi: 10.2527/jas2016.0885.
This study was designed to determine the effect of prenatal lipopolysaccharide (LPS) exposure on the postnatal metabolic response to an LPS challenge in beef heifers. Pregnant, crossbred cows ( = 50) were assigned to a prenatal immune stimulation (PIS; = 25; administered 0.1 µg/kg BW LPS subcutaneously 233 ± 15d of gestation) or saline treatment group (Control; = 25). Birth and weaning BW of calves were collected. There was not (> 0.05) a treatment × gender interaction for birth weight or 205-d adjusted weaning BW. Treatment did not affect (> 0.05) birth BW, but steers and heifers of PIS cows had greater ( < 0.02) 205-d adjusted weaning BW than offspring from Control cows. From the 2 prenatal treatment groups, heifer calves ( = 12 PIS, 11 Control) were identified at weaning (238 ± 15 d of age) to subsequently receive an LPS challenge. On d 0, heifers were fitted with indwelling jugular catheters and were moved into individual pens. On d 1, heifers (fed at 0600 h) were challenged i.v. with LPS (0.5 µg/kg BW) at 0 h (1000 h). Blood samples were collected at 30-min intervals from -2 to 8 h and again at 24 h relative to the LPS challenge. There was a treatment × time interaction ( < 0.01) for cortisol; PIS heifers had greater cortisol from 4 to 6.5 h post-LPS challenge ( < 0.001). There was a treatment × time interaction ( = 0.04) for serum glucose such that glucose was greater ( = 0.01) in PIS than Control heifers at 0.5 h, but was greater in Control than PIS heifers at 2, 4.5, and 7 h post-LPS challenge. This resulted in overall time ( < 0.01) and treatment ( < 0.01) effects such that Control heifers had greater glucose concentrations than PIS heifers. There was a tendency ( = 0.10) for a treatment × time interaction for serum NEFA, such that NEFA was greater in Control than PIS heifers at -2, -1.5, and 7 h relative to the LPS challenge ( ≤ 0.02). Also, there were time ( < 0.01) and treatment effects ( < 0.01) for NEFA with Control heifers having greater NEFA than PIS heifers. Serum BUN was affected by a treatment × time interaction ( < 0.01). Concentrations of BUN were greater in PIS heifers from -1.5 to -1 h, 1 to 2 h, at 4 h, and from 5 to 24 h relative to the LPS challenge. These results demonstrate postnatal growth and the metabolic responses of weaned beef calves can be significantly altered with a single exposure to LPS in utero.
本研究旨在确定产前脂多糖 (LPS) 暴露对肉牛小母牛产后对 LPS 挑战的代谢反应的影响。怀孕的杂交奶牛（= 50）被分配到产前免疫刺激（PIS；= 25；在妊娠 233 ± 15 天皮下给予 0.1 µg/kg BW LPS）或盐水处理组（对照；= 25）。收集了小牛的出生和断奶体重。出生体重或 205 天调整后的断奶体重没有（> 0.05）处理×性别相互作用。处理没有影响（> 0.05）出生体重，但 PIS 奶牛的公牛和小母牛的 205 天调整后的断奶体重比对照奶牛的后代更大（<0.02）。从 2 个产前处理组中，在断奶时（238 ± 15 天龄）确定小母牛（= 12 PIS，11 对照）随后接受 LPS 挑战。在 d 0，小母牛被安置带有留置颈静脉导管，并被转移到单独的围栏中。在 d 1，小母牛（在 0600 h 喂食）在 0 h（1000 h）静脉内接受 LPS（0.5 µg/kg BW）挑战。在 LPS 挑战前 2 小时至 8 小时和 24 小时时，每隔 30 分钟采集一次血液样本。皮质醇存在处理×时间交互作用（<0.01）；PIS 小母牛在 LPS 挑战后 4 至 6.5 小时的皮质醇更高（<0.001）。血清葡萄糖存在处理×时间交互作用（= 0.04），即 PIS 小母牛的葡萄糖在 0.5 小时时比对照小母牛高（= 0.01），但在 LPS 挑战后 2、4.5 和 7 小时时，对照小母牛的葡萄糖更高。这导致总体时间（<0.01）和处理（<0.01）的影响，即对照小母牛的葡萄糖浓度高于 PIS 小母牛。血清 NEFA 存在处理×时间交互作用的趋势（= 0.10），即对照小母牛在 LPS 挑战前 -2、-1.5 和 7 小时的 NEFA 高于 PIS 小母牛（≤0.02）。此外，NEFA 存在时间（<0.01）和处理效应（<0.01），对照小母牛的 NEFA 高于 PIS 小母牛。血清 BUN 受到处理×时间交互作用的影响（<0.01）。BUN 浓度在 PIS 小母牛中从 -1.5 到 -1 小时、1 到 2 小时、4 小时和 5 到 24 小时时更高，与 LPS 挑战有关。这些结果表明，产后生长和断奶肉牛小母牛的代谢反应可以通过宫内单次 LPS 暴露而显著改变。
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