J Anim Sci. 2017 Dec;95(12):5597-5605. doi: 10.2527/jas2017.1828.
Mature Simmental × Angus cows (214 cows; 635 ± 7 kg) were utilized to determine the effects of late gestation and early postpartum supplementation of methionine hydroxy analog (MHA) on cow BW, BCS, milk production, milk composition, reproduction, and calf performance until weaning in a fall-calving, cool-season grazing system. Cows were stratified by BW, age, AI sire, and assigned to 1 of 12 pastures (17 or 18 cows·pasture). Pastures were randomly allotted to 1 of 2 treatments: control (0.45 kg·cow·d of wheat midd-based pellets, = 6) or supplement including MHA (0.45 kg·cow·d of wheat midd-based pellets including 10 g MHA supplied as MFP (Novus International, Inc., St. Charles, MO; = 6). Treatments were fed 23 ± 7 d prepartum through 73 ± 7 d postpartum. Cow BW was collected at postcalving (27 ± 7 d postpartum), end of supplementation (73 ± 7 d postpartum), AI, pregnancy check, and end of trial (192 and 193 ± 7 d postpartum). At 73 ± 7 d postpartum, a subset of cow-calf pairs was used in a weigh-suckle-weigh procedure to determine milk production, and milk samples were taken to determine milk composition ( = 45·treatment). Serum from blood was collected at 73 ± 7 and 83 ± 7 d postpartum to determine cow cyclicity and concentrations of 2-hydroxy4-(methylthio) butanoic acid (HMTBa) and L-Methionine. After supplementation, all cow-calf pairs were managed as a common group until weaning (193 ± 7 d of age). Cows were bred via AI at 97 ± 7 d postpartum and clean-up bulls were turned out 11 d post-AI for a 55-d breeding season. Cows fed MHA had greater ( < 0.01) serum concentrations of HMTBa. Cow BW and BCS were not different ( ≥ 0.10) at any time points between treatments. There was no treatment effect ( ≥ 0.17) on calf birth BW, calf weaning BW (193 ± 7 d of age), or calf ADG. Calculated 24-h milk production, milk composition and component production did not differ ( ≥ 0.21). There were no differences ( ≥ 0.50) in percentage of cows cycling, AI conception rate, and overall pregnancy rate between treatments. Post-trial nutritional modeling suggests cows experienced several nutritional deficiencies beyond methionine (Met) that limited the response to Met supplementation. Although supplementation of MHA during late gestation through estimated peak lactation increased serum HMTBa concentrations, it did not affect cow performance, cow milk production, or calf performance when fall-calving cows grazed cool-season forages.
利用成熟的西门塔尔牛×安格斯牛(214 头奶牛;635±7kg),确定妊娠后期和产后早期补充蛋氨酸羟基类似物(MHA)对奶牛体重、体况评分、产奶量、乳成分、繁殖和犊牛性能的影响,直到秋季产犊、凉爽季节放牧系统中的犊牛断奶。根据体重、年龄、人工授精 sire 对奶牛进行分层,然后将其分配到 12 个牧草地中的 1 个(17 或 18 头牛·牧草地)。牧草地随机分配到 2 种处理之一:对照(0.45kg·奶牛·d 基于小麦中间的颗粒,=6)或补充物(0.45kg·奶牛·d 基于小麦中间的颗粒,包含 10g MHA 作为 MFP(Novus International,Inc.,圣查尔斯,密苏里州;=6)供应)。在产前 23±7d 至产后 73±7d 期间饲喂处理。产后(产后 27±7d)、补充结束(产后 73±7d)、人工授精、妊娠检查和试验结束(产后 192 和 193±7d)时采集奶牛体重。在产后 73±7d 时,对部分奶牛-犊牛对进行称重-哺乳-称重程序,以确定产奶量,并采集奶样以确定乳成分(=45·处理)。产后 73±7 和 83±7d 时从血液中采集血清,以确定奶牛的周期性和 2-羟基-4-(甲硫基)丁酸(HMTBa)和 L-蛋氨酸的浓度。补充后,所有奶牛-犊牛对在断奶(193±7d 龄)前作为一个共同的群体进行管理。奶牛在产后 97±7d 通过人工授精配种,产后 11d 淘汰清洁公牛进行 55d 的配种期。饲喂 MHA 的奶牛血清中 HMTBa 的浓度更高(<0.01)。在任何处理时间点,奶牛体重和体况评分均无差异(≥0.10)。在犊牛出生体重、犊牛断奶体重(193±7d 龄)或犊牛日增重方面,处理之间没有差异(≥0.17)。24h 产奶量、乳成分和成分产量无差异(≥0.21)。处理之间奶牛的周期性、人工授精受孕率和总妊娠率没有差异(≥0.50)。试验后的营养建模表明,除蛋氨酸(Met)外,奶牛还经历了几种营养缺乏,这限制了对 Met 补充的反应。尽管在妊娠后期至估计泌乳高峰期补充 MHA 增加了血清 HMTBa 浓度,但在秋季产犊的奶牛放牧凉爽季节牧草时,它并没有影响奶牛的生产性能、奶牛的产奶量或犊牛的生产性能。