Department of Biological Sciences, Marquette University, Milwaukee, WI.
Bell Museum of Natural History, University of Minnesota, Saint Paul, MN.
J Hered. 2018 May 11;109(4):462-468. doi: 10.1093/jhered/esx112.
Sex-specific genetic markers identified using restriction site-associated DNA sequencing, or RADseq, permits the recognition of a species' sex chromosome system in cases where standard cytogenetic methods fail. Thus, species with male-specific RAD markers have an XX/XY sex chromosome system (male heterogamety) while species with female-specific RAD markers have a ZZ/ZW sex chromosome (female heterogamety). Here, we use RADseq data from 5 male and 5 female South American dwarf geckos (Gonatodes humeralis) to identify an XX/XY sex chromosome system. This is the first confidently known sex chromosome system in a Gonatodes species. We used a low-coverage de novo G. humeralis genome assembly to design PCR primers to validate the male-specificity of a subset of the sex-specific RADseq markers and describe how even modest genome assemblies can facilitate the design of sex-specific PCR primers in species with diverse sex chromosome systems.
利用限制性位点相关 DNA 测序（RADseq）鉴定的性别特异性遗传标记可用于识别标准细胞遗传学方法无法识别的物种的性染色体系统。因此，具有雄性特异性 RAD 标记的物种具有 XX/XY 性染色体系统（雄性异型性），而具有雌性特异性 RAD 标记的物种具有 ZZ/ZW 性染色体（雌性异型性）。在这里，我们使用来自 5 只雄性和 5 只雌性南美侏儒壁虎（Gonatodes humeralis）的 RADseq 数据来鉴定 XX/XY 性染色体系统。这是 Gonatodes 物种中第一个被明确识别的性染色体系统。我们使用低覆盖率的从头 Gonatodes humeralis 基因组组装来设计 PCR 引物，以验证一组性别特异性 RADseq 标记的雄性特异性，并描述即使是适度的基因组组装也可以促进在具有不同性别染色体系统的物种中设计性别特异性 PCR 引物。
