Department of Biomedical Engineering, National University of Singapore, 7 Engineering Drive 1, Singapore 117574.
Lab Chip. 2018 Feb 13;18(4):574-584. doi: 10.1039/c7lc01066d.
To perform precision medicine in real-time, a sensor capable of continuously monitoring target biomolecules secreted from a patient under dynamic situations is essential. In this study, a novel portable device combining an aptamer probe and a nanofluidic component was developed, enabling the buffer-free continuous monitoring of small molecules in biological fluids. This integration is synergistic: the aptamer sensor is used to bind target biomolecules, triggering a fluorescence signal change, while the nanofluidic component is applied to achieve ion concentration polarization and convert serum into a clean buffer for aptamer signal regeneration. To demonstrate the system's versatility, we measured various adenosine triphosphate concentrations in human serum for hours with high sensitivity and specificity at minute temporal resolution. Our results demonstrate that this integrative device can be applied for the continuous measurement of target biomolecules and online signal regeneration in patient samples without the use of bulky clean buffer solutions for dynamic real-time healthcare.
为了实时进行精准医学，需要一种能够在动态情况下持续监测患者分泌的目标生物分子的传感器。在这项研究中，开发了一种新型的便携式设备，将适体探针和纳流控组件结合在一起，实现了生物流体中小分子的无缓冲连续监测。这种集成是协同的：适体传感器用于结合目标生物分子，触发荧光信号变化，而纳流控组件则用于实现离子浓度极化，并将血清转化为清洁缓冲液，以实现适体信号再生。为了证明该系统的多功能性，我们以分钟级的时间分辨率，高灵敏度和特异性，对人血清中的各种三磷酸腺苷浓度进行了数小时的测量。我们的结果表明，该集成设备可用于在无需使用大型清洁缓冲溶液的情况下，对患者样本中的目标生物分子进行连续测量和在线信号再生，从而实现动态实时医疗保健。
