在废水中培养新分离的微藻 Coelastrum sp. 以实现 CO2 固定、脂质生产和废水处理的同步进行。
Cultivation of newly isolated microalgae Coelastrum sp. in wastewater for simultaneous CO fixation, lipid production and wastewater treatment.
机构信息
Faculty of Chemical Engineering, Babol Noshirvani University of Technology, Babol, Iran.
Mazandaran Gas Company, Sari, Iran.
出版信息
Bioprocess Biosyst Eng. 2018 Apr;41(4):519-530. doi: 10.1007/s00449-017-1887-7. Epub 2018 Jan 3.
Cultivation of microalgae in wastewater is a promising and cost-effective approach for both CO biofixation and wastewater remediation. In this study, a new strain of Coelastrum sp. was isolated from cattle manure leachate. The isolated microalgae were then cultivated in wastewater. Effects of different sCOD concentrations (600, 750, 900, 1050 mg L) and light intensities (1000, 2300, 4600, 6900 and 10000 Lux) on biomass production, CO consumption rate and nutrient removal from wastewater were investigated. The results showed that maximum cell growth and CO consumption rate were 2.71 g L and 53.12 mg L day, respectively, which were obtained in the wastewater with 750 mg L sCOD and under the light intensity of 6900 Lux. The microalgae were able to completely consume all CO after incubation period of 4 days. The highest sCOD, total Kjeldahl nitrogen (TKN), nitrate and total phosphorous (TP) removal at such conditions were 53.45, 91.18, 87.51 and 100%, respectively. The lipid content of microalgal biomass was also measured under different light intensities; maximum amount of lipid was determined to be 50.77% under illumination of 2300 Lux. Finally, the CO consumption rate and biomass productivity of microalgae in semi-batch culture with continuous gas flow (CO 6%:N 94%) were investigated. The rate of CO consumption and biomass productivity were 0.528 and 0.281 g L day, respectively. The TKN, nitrate, TP and sCOD removal rate of microalgae were 83.51, 80.91, 100, 41.4%, respectively.
利用废水培养微藻是一种很有前景且具有成本效益的方法，既能进行 CO 固定，又能修复废水。本研究从牛粪渗滤液中分离出一株新的胶球藻。然后，将分离出的微藻在废水中培养。考察了不同 sCOD 浓度（600、750、900、1050mg/L）和光照强度（1000、2300、4600、6900 和 10000Lux）对生物量生产、CO 消耗速率和废水中养分去除的影响。结果表明，在 sCOD 为 750mg/L、光照强度为 6900Lux 的废水中，细胞生长和 CO 消耗率最高，分别为 2.71g/L 和 53.12mg/L·d。在培养 4 天后，微藻能够完全消耗所有 CO。在这些条件下，sCOD、总凯氏氮（TKN）、硝酸盐和总磷（TP）的去除率最高，分别为 53.45%、91.18%、87.51%和 100%。还在不同光照强度下测量了微藻生物质的脂质含量；在 2300Lux 的光照下，确定了最大脂质含量为 50.77%。最后，研究了连续气流（CO 6%：N 94%）条件下半分批培养中微藻的 CO 消耗速率和生物量生产力。CO 消耗速率和生物量生产力分别为 0.528 和 0.281g/L·d。微藻对 TKN、硝酸盐、TP 和 sCOD 的去除率分别为 83.51%、80.91%、100%和 41.4%。
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