Yuan Shih-Chang, Chin Shih-Wen, Lee Chen-Yu, Chen Fure-Chyi
Department of Plant Industry, National Pingtung University of Science & Technology, Pingtung, 91201, Taiwan.
Bot Stud. 2018 Jan 3;59(1):1. doi: 10.1186/s40529-017-0218-2.
In a breeding program, usually only superior parents are chosen for cross hybridization. Pollens of elite cultivars may not be available at hand. Properly stored pollens provide an opportunity for cross hybridization at unavailable time.
Pollen of a Phalaenopsis hybrid was evaluated for the storage ability at different temperatures, including room temperature, 4, - 20, and - 80 °C for up to 96 weeks. The viability of pollen was assessed by TTC staining, in vitro germination and hand pollination during and after storage. Pollen stored at all temperatures for 4 weeks remained viable and capable of successful pollination. Pollen lost its viability after 4 weeks at room temperature. Pollen remains viable after 40 weeks at 4 °C, and after 96 weeks at both - 20 and - 80 °C of storage. Viable pollen could be successfully pollinated to the female parent at all effective storage conditions and produced seeds.
Our results indicate that Phalaenopsis pollen can be stored at 4 °C up to 40 weeks for short-term purpose. For long-term storage, pollen can be kept at both - 20 and -80 °C.
在育种计划中，通常仅选择优良亲本进行杂交。手头可能没有优良品种的花粉。妥善储存的花粉为在无法获取花粉时进行杂交提供了机会。
对一种蝴蝶兰杂交种的花粉在不同温度下（包括室温、4℃、-20℃和-80℃）长达96周的储存能力进行了评估。通过TTC染色、体外萌发以及储存期间和之后的人工授粉来评估花粉的活力。在所有温度下储存4周的花粉仍保持活力并能够成功授粉。在室温下4周后花粉失去活力。在4℃下储存40周后以及在-20℃和-80℃下储存96周后花粉仍保持活力。在所有有效的储存条件下，有活力的花粉都能成功授粉给母本并产生种子。
我们的结果表明，蝴蝶兰花粉可在4℃下短期储存长达40周。对于长期储存，花粉可保存在-20℃和-80℃。
