Jiang Yan, Qi Hui, Zhang Xian M
a Engineering Research Centre for Waste Oil Recovery Technology and Equipment , Ministry Education, Chongqing Technology and Business University , Chongqing , P R China.
b College of Foreign Languages , Chongqing Technology and Business University , Chongqing , P R China.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2018 Apr 16;53(5):448-456. doi: 10.1080/10934529.2017.1409579. Epub 2018 Jan 4.
NAP (Naphthalene) and ANT (anthracene) usually co-exist in environment and possessed interactional effects on their biodegradation in environment. Presently, a strain of Acinetobacter johnsonii was employed to degrade NAP and ANT in single- and dual-substrate systems. NAP was utilized as prefer substrate by cells to accelerate ANT biodegradation. As much as 200 mg L ANT could be entirely degraded with 1,500 mg L NAP, which was beyond bacterial potential in single substrate system. Especially, the shortest biodegradation period (103 h) for ANT was observed with the presence of 50 mg L NAP. By contrast, ANT showed strong inhibition on NAP degradation, while the peak biodegradation of 1,950 mg L NAP with 50 mg L ANT could still proceed. By introducing an inhibition constant parameter to fit the inhibition on cells, modeling indicated the substrate inhibition for NAP and ANT over the concentrations of 174 and 49 mg L, respectively. Furthermore, enzyme assay revealed the pathway of meta fission in NAP biodegradation due to the appearance of catechol 2,3-dioxygenase activity, and low-level lipase excretion was also found in both NAP and ANT biodegradation, but hardly affect NAP and ANT biodegradation in the present study. To research the interplay of NAP and ANT is conducive to targeted decontamination.
萘（NAP）和蒽（ANT）通常共存于环境中，且对其在环境中的生物降解具有相互作用。目前，一株约翰逊不动杆菌被用于在单底物和双底物系统中降解NAP和ANT。细胞将NAP用作优先底物以加速ANT的生物降解。在存在1500 mg/L NAP的情况下，高达200 mg/L的ANT能够被完全降解，这超出了细菌在单底物系统中的潜力。特别是，在存在50 mg/L NAP的情况下，观察到ANT的最短生物降解期（103小时）。相比之下，ANT对NAP的降解表现出强烈抑制作用，而在存在50 mg/L ANT的情况下，1950 mg/L NAP的生物降解峰值仍可进行。通过引入抑制常数参数来拟合对细胞的抑制作用，模型表明NAP和ANT分别在浓度超过174和49 mg/L时对底物产生抑制。此外，酶活性测定揭示了由于儿茶酚2,3-双加氧酶活性的出现，NAP生物降解中的间位裂解途径，并且在NAP和ANT生物降解过程中均发现了低水平的脂肪酶分泌，但在本研究中几乎不影响NAP和ANT的生物降解。研究NAP和ANT的相互作用有助于进行有针对性的去污处理。
