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猪α8干扰素（IFNα8）的种属特异性抗病毒活性

Species Specific Antiviral Activity of Porcine Interferon-α8 (IFNα8).

作者信息

Kim Eunhye, Jhun Hyunjhung, Kim Joohee, Park Unjoo, Jo Seunghyun, Kwak Areum, Kim Sinae, Nguyen Tam T, Kang Yongsun, Choi Insoo, Lee Joongbok, Kim Heijun, Kim Younghyun, Lee Siyoung, Kim Soohyun

机构信息

Laboratory of Cytokine Immunology, Department of Biomedical Science and Technology, Konkuk University, Seoul 05029, Korea.

Collage of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.

出版信息

Immune Netw. 2017 Dec;17(6):424-436. doi: 10.4110/in.2017.17.6.424. Epub 2017 Dec 19.

DOI:10.4110/in.2017.17.6.424
PMID:29302255
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5746612/
Abstract

Interferons (IFNs) have been known as antiviral genes and they are classified by type 1, type 2, and type 3 IFN. The type 1 IFN consists of IFNα, IFNβ, IFNτ, and IFNω whereas the type 2 IFN consists of only IFNγ, which is a key cytokine driving T helper cell type 1 immunity. IFNλ belongs to the type 3 IFN, which is also known as IL-28 and IL-29 possessing antiviral activities. Type 1 IFN is produced by viral infection whereas type 2 IFN is induced by mitogenic or antigenic T-cell stimuli. The IFNτ of bovine was first discovered in an ungulate ruminant recognition hormone. IFNτ belongs to the type 1 IFN with the common feature of type 1 IFN such as antiviral activity. IFNs have been mostly studied for basic research and clinical usages therefore there was no effort to investigate IFNs in industrial animals. Here we cloned porcine IFNα8 from peripheral blood mononuclear cells of Korean domestic pig (). The newly cloned IFNα8 amino acid sequence from Korean domestic pig shares 98.4% identity with the known porcine IFNα8 in databank. The recombinant porcine IFNα8 showed potent antiviral activity and protected bovine Madin-Darby bovine kidney epithelial (MDBK) cells from the cytopathic effect of vesicular stomatitis virus, but it failed to protect human Wistar Institute Susan Hayflick (WISH) cells and canine Madin-Darby canine kidney epithelial-like (MDCK) cells. The present study demonstrates species specific antiviral activity of porcine IFNα8.

摘要

干扰素（IFNs）一直被认为是抗病毒基因，它们被分为1型、2型和3型干扰素。1型干扰素由IFNα、IFNβ、IFNτ和IFNω组成，而2型干扰素仅由IFNγ组成，IFNγ是驱动1型辅助性T细胞免疫的关键细胞因子。IFNλ属于3型干扰素，也被称为具有抗病毒活性的IL-28和IL-29。1型干扰素由病毒感染产生，而2型干扰素由促有丝分裂或抗原性T细胞刺激诱导产生。牛的IFNτ最初是在一种有蹄类反刍动物识别激素中发现的。IFNτ属于1型干扰素，具有1型干扰素的共同特征，如抗病毒活性。干扰素大多用于基础研究和临床应用，因此没有对家畜中的干扰素进行研究。在这里，我们从韩国本土猪的外周血单个核细胞中克隆了猪IFNα8。从韩国本土猪新克隆的IFNα8氨基酸序列与数据库中已知的猪IFNα8有98.4%的同一性。重组猪IFNα8显示出强大的抗病毒活性，可保护牛肾上皮细胞（MDBK）免受水疱性口炎病毒的细胞病变作用，但未能保护人胚肺成纤维细胞（WISH）和犬肾上皮样细胞（MDCK）。本研究证明了猪IFNα8的种属特异性抗病毒活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f803/5746612/215c0465d600/in-17-424-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f803/5746612/4916d227431b/in-17-424-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f803/5746612/56f36510e163/in-17-424-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f803/5746612/8133cbcea53c/in-17-424-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f803/5746612/d8a8f634f2de/in-17-424-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f803/5746612/2326043188b5/in-17-424-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f803/5746612/cdbaf90da456/in-17-424-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f803/5746612/215c0465d600/in-17-424-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f803/5746612/4916d227431b/in-17-424-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f803/5746612/56f36510e163/in-17-424-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f803/5746612/8133cbcea53c/in-17-424-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f803/5746612/d8a8f634f2de/in-17-424-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f803/5746612/2326043188b5/in-17-424-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f803/5746612/cdbaf90da456/in-17-424-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f803/5746612/215c0465d600/in-17-424-g007.jpg

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