Park Jisang, Choe Chung-Hyeon, Kim Ju, Yang Jing Shian, Kim Jin Hyun, Jang Hyonseok, Jang Yong-Suk
Department of Bioactive Material Sciences and Institute of Bioactive Materials, Chonbuk National University, Jeonju 54896, Korea.
NBM Inc., Wanju 55322, Korea.
Immune Netw. 2017 Dec;17(6):437-450. doi: 10.4110/in.2017.17.6.437. Epub 2017 Dec 19.
Major histocompatibility complex (MHC) class II molecules, which are recognized for their primary function of presenting an antigen to the T cell receptor, are involved in various signaling pathways in B cell activation. We identified heterogeneous nuclear ribonucleoprotein (hnRNP) A2B1 as an MHC class II molecule-associated protein involved in MHC class II-mediated signal transduction in lipopolysaccharide (LPS)-stimulated 38B9 B cells. Although the function of hnRNP A2B1 in the nucleus is primarily known, the level of hnRNP A2B1 in the cytoplasm was increased in LPS-stimulated 38B9 cells, while it was not detected in the cytoplasm of non-treated 38B9 cells. The silencing of hnRNP A2B1 expression using siRNA disturbed B cell maturation by regulation of mitogen-activated protein kinase signaling, NF-κB activation, and protein kinase B activation. These results suggest that hnRNP A2B1 is associated with MHC class II molecules and is involved in B cell activation signaling pathways in LPS-stimulated 38B9 cells.
主要组织相容性复合体（MHC）II类分子以其向T细胞受体呈递抗原的主要功能而闻名，它参与B细胞活化的各种信号通路。我们鉴定出异质性核核糖核蛋白（hnRNP）A2B1是一种与MHC II类分子相关的蛋白质，它参与脂多糖（LPS）刺激的38B9 B细胞中MHC II类介导的信号转导。尽管hnRNP A2B1在细胞核中的功能已为人所知，但在LPS刺激的38B9细胞中，细胞质中hnRNP A2B1的水平升高，而在未处理的38B9细胞的细胞质中未检测到。使用小干扰RNA（siRNA）沉默hnRNP A2B1的表达，通过调节丝裂原活化蛋白激酶信号传导、核因子κB（NF-κB）激活和蛋白激酶B激活，干扰了B细胞的成熟。这些结果表明，hnRNP A2B1与MHC II类分子相关，并参与LPS刺激的38B9细胞中的B细胞活化信号通路。
