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sp. kr6产生的抗氧化色素的提取与部分特性分析

Extraction and partial characterisation of antioxidant pigment produced by sp. kr6.

作者信息

Jiménez Maria Elisa Pailliè, Pinilla Cristian Mauricio Barreto, Rodrigues Eliseu, Brandelli Adriano

机构信息

a Laboratório de Bioquímica e Microbiologia Aplicada , Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul , Porto Alegre , Brazil.

出版信息

Nat Prod Res. 2019 Jun;33(11):1541-1549. doi: 10.1080/14786419.2017.1423304. Epub 2018 Jan 5.

Abstract

Pigments synthesised by sp. kr6 growing on feather waste were extracted and characterised. The pigment extract was characterised by KOH test, UV-vis, CIELAB colour system, HPLC-DAD-MS, FTIR and its antioxidant capacity was evaluated. A positive bathochromic shift was observed when kr6 colonies or pigment extracts were subjected to alkaline solution (20% KOH) and a at 450 nm was detected for acetone extracts, although no typical fine structure of carotenoids was detected in the electomagnetic spectra. The HPLC profile of the extracted pigment showed that the compound has three different peaks with near 450 nm. The FTIR analysis shows some principal functional groups from a flexirubin-like molecule. The pigmented compound also presents antioxidant activity evaluated by the scavenging of the ABTS radical.

摘要

对生长在羽毛废弃物上的sp. kr6合成的色素进行了提取和表征。通过KOH试验、紫外-可见光谱、CIELAB颜色系统、高效液相色谱-二极管阵列-质谱联用仪、傅里叶变换红外光谱对色素提取物进行了表征,并评估了其抗氧化能力。当kr6菌落或色素提取物用碱性溶液(20% KOH)处理时,观察到正向红移,丙酮提取物在450 nm处有吸收峰,尽管在电磁光谱中未检测到典型的类胡萝卜素精细结构。提取色素的高效液相色谱图显示该化合物有三个不同的峰,在450 nm附近有吸收。傅里叶变换红外光谱分析显示了来自类弹性红素样分子的一些主要官能团。通过ABTS自由基清除法评估,该色素化合物还具有抗氧化活性。

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