Cottrell G A, Price D A, Doble K E, Hettle S, Sommerville J, MacDonald M
Biol Bull. 1992 Aug;183(1):113-122. doi: 10.2307/1542412.
Extracts were prepared from selected neurons taken from the central ganglia of the pulmonate snail Helix aspersa, and parallel radioimmunoassay (RIA) and high pressure liquid chromatography (HPLC) were used to analyze for FMRFamide-related peptides (FaRPs). Some neurons (e.g., the cerebral C3 neuron) contained only the tetrapeptides related to FMRFamide (tetra-FaRPs), whereas two clusters of neurons in the parietal ganglia contain a preponderance of the hepta-FaRPs. Using cloned cDNA probes in molecular hybridization studies, we showed that the C3 neuron (and some other large neurons) contain the mRNA species that encodes the tetra-FaRPs. In contrast, the neurons of the parietal clusters predominantly express the mRNA that encodes the hepta-FaRPs. These results were confirmed by in situ hybridization with antisense RNA on whole mount ganglia. Each type of experiment supports the view that FaRP-containing Helix neurons express either the tetra-FaRPs or the hepta-FaRPs, but not both.
从取自肺螺亚纲蜗牛盖罩大蜗牛中枢神经节的特定神经元中提取样本，并采用平行放射免疫分析（RIA）和高压液相色谱法（HPLC）分析FMRF酰胺相关肽（FaRPs）。一些神经元（如脑C3神经元）仅含有与FMRF酰胺相关的四肽（四肽-FaRPs），而壁神经节中的两簇神经元则主要含有七肽-FaRPs。在分子杂交研究中使用克隆的cDNA探针，我们发现C3神经元（以及其他一些大神经元）含有编码四肽-FaRPs的mRNA种类。相比之下，壁神经节簇中的神经元主要表达编码七肽-FaRPs的mRNA。这些结果通过在整装神经节上用反义RNA进行原位杂交得到了证实。每种类型的实验都支持这样一种观点，即含有FaRP的盖罩大蜗牛神经元要么表达四肽-FaRPs，要么表达七肽-FaRPs，但不会同时表达两者。
