Takeno T, Li S S
Laboratory of Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
Biochem J. 1989 Feb 1;257(3):921-4. doi: 10.1042/bj2570921.
Human genomic clones containing parts of the lactate dehydrogenase B (LDH-B) gene (approx. 25 kb in length) were isolated and characterized. The protein-coding sequence of human LDH-B gene is interrupted by six introns at codons nos. 42-43, 82, 140, 198, 237 and 278-279, and the positions of these introns are homologous to those of LDH-A genes from man and mouse. The 5' non-coding region of human LDH-B gene is interrupted by an intron six nucleotide residues upstream of the ATG translation-initiation site, whereas those of human and mouse LDH-A genes are interrupted at 24 nucleotide residues 5' to the ATG initiation codon. As is the case of LDH-A genes from man and mouse, there is no intron in the 3' non-coding region of human LDH-B gene.
分离并鉴定了包含乳酸脱氢酶B(LDH-B)基因部分片段(长度约25kb)的人类基因组克隆。人类LDH-B基因的蛋白质编码序列在第42-43、82、140、198、237和278-279号密码子处被六个内含子打断,这些内含子的位置与人及小鼠的LDH-A基因的内含子位置同源。人类LDH-B基因的5'非编码区在ATG翻译起始位点上游六个核苷酸残基处被一个内含子打断,而人类和小鼠LDH-A基因的5'非编码区在ATG起始密码子5'端24个核苷酸残基处被打断。与人类和小鼠的LDH-A基因情况一样,人类LDH-B基因的3'非编码区没有内含子。