线虫秀丽隐杆线虫的乳酸脱氢酶基因在其编码序列中仅含有六个内含子中的两个，而这六个内含子在鸟类和哺乳动物的乳酸脱氢酶基因的蛋白质编码序列中是保守的。

The lactate dehydrogenase gene from nematode Caenorhabditis elegans contains only two of six introns conserved in the protein-encoding sequence of LDH genes from bird and mammals.

作者信息

Mannen H, Li S S

机构信息

Laboratory of Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA.

出版信息

Biochem Mol Biol Int. 1995 Dec;37(6):1057-61.

PMID:8747535

Abstract

The protein-encoding region of L-lactate dehydrogenase (LDH) gene from nematode, Caenorhabditis elegans, was amplified by polymerase-chain-reaction from total genomic DNA and its nucleotide sequence determined. A comparison of this genomic sequence with the published sequence of nematode LDH cDNA reveals the presence of two introns of 57 and 47 nucleotides at codon no. 82 and 279-280, respectively. The positions of the two introns present in this invertebrate LDH gene correspond to the second and sixth introns of vertebrate LDH genes. The protein-coding sequence of human LDH-A (muscle), LDH-B (heart) and LDH-C (testis), mouse LDH-A, and duck LDH-B genes has previously been shown to be interrupted by six introns at the homologous positions.

摘要

通过聚合酶链反应从线虫秀丽隐杆线虫的总基因组DNA中扩增出线虫L-乳酸脱氢酶（LDH）基因的蛋白质编码区，并测定其核苷酸序列。将该基因组序列与已发表的线虫LDH cDNA序列进行比较，发现在第82位密码子和第279 - 280位密码子处分别存在两个长度为57和47个核苷酸的内含子。该无脊椎动物LDH基因中存在的两个内含子的位置与脊椎动物LDH基因的第二个和第六个内含子相对应。先前已证明人类LDH - A（肌肉）、LDH - B（心脏）和LDH - C（睾丸）、小鼠LDH - A以及鸭LDH - B基因的蛋白质编码序列在同源位置被六个内含子中断。