Mannen H, Li S S
Laboratory of Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA.
Biochem Mol Biol Int. 1995 Dec;37(6):1057-61.
The protein-encoding region of L-lactate dehydrogenase (LDH) gene from nematode, Caenorhabditis elegans, was amplified by polymerase-chain-reaction from total genomic DNA and its nucleotide sequence determined. A comparison of this genomic sequence with the published sequence of nematode LDH cDNA reveals the presence of two introns of 57 and 47 nucleotides at codon no. 82 and 279-280, respectively. The positions of the two introns present in this invertebrate LDH gene correspond to the second and sixth introns of vertebrate LDH genes. The protein-coding sequence of human LDH-A (muscle), LDH-B (heart) and LDH-C (testis), mouse LDH-A, and duck LDH-B genes has previously been shown to be interrupted by six introns at the homologous positions.
通过聚合酶链反应从线虫秀丽隐杆线虫的总基因组DNA中扩增出线虫L-乳酸脱氢酶（LDH）基因的蛋白质编码区，并测定其核苷酸序列。将该基因组序列与已发表的线虫LDH cDNA序列进行比较，发现在第82位密码子和第279 - 280位密码子处分别存在两个长度为57和47个核苷酸的内含子。该无脊椎动物LDH基因中存在的两个内含子的位置与脊椎动物LDH基因的第二个和第六个内含子相对应。先前已证明人类LDH - A（肌肉）、LDH - B（心脏）和LDH - C（睾丸）、小鼠LDH - A以及鸭LDH - B基因的蛋白质编码序列在同源位置被六个内含子中断。
