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人微粒体谷胱甘肽S-转移酶。其在六氯丁二烯与谷胱甘肽结合反应中的作用。

Human microsomal glutathione S-transferase. Its involvement in the conjugation of hexachlorobuta-1,3-diene with glutathione.

作者信息

McLellan L I, Wolf C R, Hayes J D

机构信息

University Department of Clinical Chemistry, Royal Infirmary, Edinburgh, Scotland, U.K.

出版信息

Biochem J. 1989 Feb 15;258(1):87-93. doi: 10.1042/bj2580087.

DOI:10.1042/bj2580087
PMID:2930518
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1138327/
Abstract

A microsomal glutathione S-transferase (GST) was purified from human liver. This enzyme was shown to have characteristics similar to those of the rat microsomal GST described by Morgenstern & De Pierre [(1983) Eur. J. Biochem. 134, 591-597]. The specific activity of human microsomal GST towards 1-chloro-2,4-dinitrobenzene or cumene hydroperoxide can be stimulated by treating the enzyme with N-ethylmaleimide. This enhancement of activity is accompanied by increased sensitivity to inhibition by haematin and cholic acid. The subunit Mr values of the rat and human enzymes are similar (approx. 17,300), and the proteins are immunologically related. During purification, both human and rat microsomal GST enzymes are the only hepatic proteins obtained from Triton X-100-solubilized microsomal fractions that show activity towards the nephrotoxin hexachlorobuta-1,3-diene. The involvement of microsomal GST in toxification reactions is discussed.

摘要

从人肝脏中纯化出一种微粒体谷胱甘肽S-转移酶（GST）。已证明该酶具有与Morgenstern和De Pierre所描述的大鼠微粒体GST相似的特性[（1983年）《欧洲生物化学杂志》134卷，591 - 597页]。用人微粒体GST对1-氯-2,4-二硝基苯或氢过氧化异丙苯的比活性可通过用N-乙基马来酰亚胺处理该酶来刺激。活性的这种增强伴随着对血红素和胆酸抑制的敏感性增加。大鼠和人酶的亚基相对分子质量值相似（约17,300），并且这两种蛋白质在免疫学上相关。在纯化过程中，人和大鼠微粒体GST酶是从Triton X-100增溶的微粒体组分中获得的仅有的对肾毒素六氯丁二烯1,3-二烯有活性的肝脏蛋白质。讨论了微粒体GST在解毒反应中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2aa/1138327/7b37d8d34c8a/biochemj00213-0098-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2aa/1138327/b1a8004a2501/biochemj00213-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2aa/1138327/7b37d8d34c8a/biochemj00213-0098-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2aa/1138327/b1a8004a2501/biochemj00213-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2aa/1138327/7b37d8d34c8a/biochemj00213-0098-b.jpg

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Assays for differentiation of glutathione S-transferases.谷胱甘肽S-转移酶的鉴别测定
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Distribution of glutathione S-transferase isoenzymes in human kidney: basis for possible markers of renal injury.谷胱甘肽S-转移酶同工酶在人肾中的分布：肾损伤潜在标志物的基础
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Eur J Biochem. 1983 Aug 15;134(3):591-7. doi: 10.1111/j.1432-1033.1983.tb07607.x.
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A set of inhibitors for discrimination between the basic isozymes of glutathione transferase in rat liver.一组用于区分大鼠肝脏中谷胱甘肽转移酶基本同工酶的抑制剂。
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J Pharmacol Exp Ther. 1984 Jan;228(1):202-8.