从藏牦牛中分离的植物乳杆菌的抗菌活性。
Antibacterial activity of Lactobacillus plantarum isolated from Tibetan yaks.
机构信息
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; University College of Veterinary & Animal Sciences, The Islamia University of Bahawalpur, 63100 Pakistan.
出版信息
Microb Pathog. 2018 Feb;115:293-298. doi: 10.1016/j.micpath.2017.12.077. Epub 2018 Jan 3.
The main purpose of our study was to isolate the Lactobacillus strains from Tibetan yaks, which may have antibacterial activity. Three Lactobacillus plantarum (L. plantarum) strains named LP1, LP2, LP3 and one Pediococcus pentosaceus (P. pentosaceus) named PT2 were isolated from fecal samples of Tibetan yaks. We regarded indigenous Escherichia coli (E. coli ATCC 25922) and Staphylococcus aureus (S. aureus ATCC 25923) as indicator pathogens. The Bacteriocins derived from these isolated strains manifested high antibacterial activity against E. coli, the highest activity was observed in the case of LP1. On the other hand, PT2 had the most powerful bacteriostatic ability against S. aureus. In addition, all strains were positive for Ent A, which plays an important role in secreting pediocin-like bacteriocins and shows potential probiotic traits of bile and low PH tolerance. Overall, L. plantarum strains in present study could be employed as effective antibacterial substances and can help to remit the problem of antibiotic resistance in veterinary medicine.
我们研究的主要目的是从藏牦牛粪便样本中分离出具有抗菌活性的乳酸菌菌株。从藏牦牛粪便样本中分离出 3 株植物乳杆菌（L. plantarum）菌株，分别命名为 LP1、LP2 和 LP3，以及 1 株戊糖片球菌（P. pentosaceus）菌株，命名为 PT2。我们以本土大肠杆菌（E. coli ATCC 25922）和金黄色葡萄球菌（S. aureus ATCC 25923）作为指示病原体。这些分离株产生的细菌素对大肠杆菌表现出很高的抗菌活性，其中 LP1 的活性最高。另一方面，PT2 对金黄色葡萄球菌的抑菌能力最强。此外，所有菌株均为 Ent A 阳性，Ent A 在分泌类肠球菌素方面发挥着重要作用，表现出潜在的胆汁耐受和低 PH 值耐受的益生菌特性。总的来说，本研究中的植物乳杆菌菌株可以作为有效的抗菌物质，有助于缓解兽医领域的抗生素耐药问题。
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