Nicoloso M, Liang H Q, Bachellerie J P
Centre de Recherches de Biochimie et Génétique Cellulaires du CNRS, Université Paul-Sabatier, Toulouse, France.
Biochem Biophys Res Commun. 1989 Mar 31;159(3):1233-41. doi: 10.1016/0006-291x(89)92242-0.
A dot-blot hybridization procedure with synthetic oligonucleotide probes is reported, which allows the quantitative titration in genomic DNA of variant forms of repeated genes differing by a single nucleotide change. It involves the utilization of a pair of 22-base long oligonucleotides matching the two variant sequences and the choice of an hybridization temperature very close to the Td of the oligonucleotide/DNA duplexes. The selectivity is achieved through a competition between the cognate labeled and the non-cognate unlabeled probes in the hybridization mixture.
报道了一种使用合成寡核苷酸探针的斑点杂交程序，该程序可对因单个核苷酸变化而不同的重复基因变体形式进行基因组DNA定量滴定。它涉及使用一对与两个变体序列匹配的22个碱基长的寡核苷酸，并选择非常接近寡核苷酸/DNA双链体解链温度（Td）的杂交温度。选择性是通过杂交混合物中同源标记探针和非同源未标记探针之间的竞争来实现的。
