School of Life Sciences, Nanchang University, Nanchang, Jiangxi 330031, PR China; Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, PR China.
School of Life Sciences, Nanchang University, Nanchang, Jiangxi 330031, PR China.
Microb Pathog. 2018 Feb;115:299-303. doi: 10.1016/j.micpath.2017.12.080. Epub 2018 Jan 3.
Human respiratory syncytial virus (RSV) can cause severe acute lower respiratory tract disease leading to numerous hospitalizations and deaths in the infant and elderly populations worldwide, while no vaccine or effective drug is available for RSV infections. In the present study, truncated G protein was successfully expressed both in prokaryotic and eukaryotic system, and high levels of serum IgG in response to truncated G protein were observed both in GD-protein group (intramuscularly with purified GD protein) and GD-VNP20009 group (challenged via the oral route with 1 × 10 CFU of pLIVE-RSV-GD-VNP20009 strains) since 21th day, and GD-VNP20009 significantly reduced the productions of IL-1β, IL-6, and TNF-α, histamine and pathological features caused by the RSV Long strain (P < .01). Our data indicated that Salmonella typhimurium can be used to deliver truncated G DNA vaccine and represents a promising effect to protect host against RSV.
人呼吸道合胞病毒（RSV）可引起严重的急性下呼吸道疾病，导致全球婴儿和老年人大量住院和死亡，而目前尚无针对 RSV 感染的疫苗或有效药物。在本研究中，截短 G 蛋白在原核和真核系统中均成功表达，并且在 GD-蛋白组（肌肉内给予纯化的 GD 蛋白）和 GD-VNP20009 组（通过口服途径用 1×10 CFU 的 pLIVE-RSV-GD-VNP20009 株进行攻击）中均观察到针对截短 G 蛋白的血清 IgG 水平升高，自第 21 天起，并且 GD-VNP20009 显著降低了 RSV Long 株引起的 IL-1β、IL-6 和 TNF-α、组胺和组织病理学特征（P<0.01）。我们的数据表明，鼠伤寒沙门氏菌可用于递送截短 G DNA 疫苗，对保护宿主免受 RSV 感染具有有前途的效果。
