Hopkins G J, Barter P J
Baker Medical Research Institute, Melbourne, Victoria, Australia.
Atherosclerosis. 1989 Jan;75(1):73-82. doi: 10.1016/0021-9150(89)90209-8.
We have examined the effect of added apolipoprotein A-I (apoA-I) on the changes in high density lipoprotein (HDL) particle size that occur when human plasma is incubated in vitro. In the absence of added apoA-I, incubation of plasma at 37 degrees C resulted in a dramatic increase in HDL particle size. When these incubations contained an inhibitor of LCAT, an additional population of smaller HDL particles was formed. These changes in particle size were even more pronounced when the incubations were supplemented with an artificial triglyceride emulsion, Intralipid. All these changes in HDL particle size were markedly inhibited when incubations were supplemented with apoA-I. Even when the amount of added apoA-I was as little as 4.5% of the endogenous apolipoprotein there was an obvious inhibition of the changes in HDL particle size. The presence of added apoA-I sufficient to increase the plasma concentration by 18% virtually abolished the changes in HDL particle size. This effect did not relate to an inhibition of cholesterol esterification, nor did it appear to depend on an incorporation of the added apoA-I into the HDL.
我们研究了添加载脂蛋白A-I（apoA-I）对人血浆在体外孵育时高密度脂蛋白（HDL）颗粒大小变化的影响。在不添加apoA-I的情况下，血浆在37℃孵育会导致HDL颗粒大小显著增加。当这些孵育体系中含有卵磷脂胆固醇酰基转移酶（LCAT）抑制剂时，会形成另外一群较小的HDL颗粒。当孵育体系中添加人工甘油三酯乳剂英脱利匹特时，颗粒大小的这些变化更加明显。当孵育体系中添加apoA-I时，HDL颗粒大小的所有这些变化均受到显著抑制。即使添加的apoA-I量低至内源性载脂蛋白的4.5%，HDL颗粒大小的变化也会受到明显抑制。添加足以使血浆浓度增加18%的apoA-I实际上消除了HDL颗粒大小的变化。这种作用与胆固醇酯化的抑制无关，似乎也不依赖于将添加的apoA-I掺入HDL中。
