Guaragna-Filho Guilherme, Calixto Antônio Ramos, De Paula Georgette Beatriz, De Oliveira Laurione Cândido, Morcillo André Moreno, De Mello Maricilda Palandi, Maciel-Guerra Andrea Trevas, Guerra-Junior Gil
Interdisciplinary Group for Study of Sex Determination and Differentiation (GIEDDS), Growth and Development Laboratory, Center for Investigation in Pediatrics (CIPED), School of Medicine, State University of Campinas (UNICAMP),R. Tessalia Vieira de Camargo, 126, Campinas, São Paulo 13083-887, Brazil.
Laboratory of Investigation in Metabolism and Diabetes (LIMED), UNICAMP, Campinas, São Paulo, Brazil.
J Pediatr Endocrinol Metab. 2018 Jan 26;31(2):191-194. doi: 10.1515/jpem-2017-0351.
Inhibin B is a hormone produced by the Sertoli cells that can provide important information for the investigation of disorders of sex development (DSD) with 46,XY karyotype. The aim of this study is to compare two enzyme-linked immunosorbent assay (ELISA) assays for dosage of serum inhibin B in patients with 46,XY DSD with normal testosterone secretion.
Twenty-nine patients with 46,XY DSD and normal testosterone secretion (partial androgen insensitivity syndrome [PAIS] [n=8]; 5α-reductase deficiency [n=7] and idiopathic 46,XY DSD [n=14]) were included. Molecular analysis of the AR and SRD5A2 genes were performed in all patients and the NR5A1 gene analysis in the idiopathic group. Measurements of inhibin B were performed by two second-generation ELISA assays (Beckman-Coulter and AnshLabs). Assays were compared using the interclass correlation coefficient (ICC) and the Bland-Altman method.
ICC was 0.915 [95% confidence interval (CI): 0.828-0.959], however, a discrepancy was observed between trials, which is more evident among higher values when analyzed by the Bland-Altman method.
It is recommended to perform the inhibin B measurement always using the same ELISA kit when several evaluations are required for a specific patient.
抑制素B是由支持细胞产生的一种激素，可为46,XY核型性发育障碍（DSD）的研究提供重要信息。本研究的目的是比较两种酶联免疫吸附测定（ELISA）方法检测睾酮分泌正常的46,XY DSD患者血清抑制素B的剂量。
纳入29例睾酮分泌正常的46,XY DSD患者（部分雄激素不敏感综合征[PAIS][n = 8]；5α-还原酶缺乏症[n = 7]和特发性46,XY DSD[n = 14]）。对所有患者进行AR和SRD5A2基因的分子分析，对特发性组进行NR5A1基因分析。采用两种第二代ELISA方法（贝克曼库尔特公司和安赛公司）检测抑制素B。使用组内相关系数（ICC）和布兰德-奥特曼方法对检测方法进行比较。
ICC为0.915[95%置信区间（CI）：0.828 - 0.959]，然而，试验之间存在差异，用布兰德-奥特曼方法分析时，在较高值之间差异更明显。
对于特定患者需要进行多次评估时，建议始终使用同一ELISA试剂盒检测抑制素B。
