College of Life Science, Shandong Agricultural University, Taian 271018, PR China.
College of Life Science, Shandong Agricultural University, Taian 271018, PR China.
Int J Biol Macromol. 2018 May;111:121-128. doi: 10.1016/j.ijbiomac.2018.01.010. Epub 2018 Jan 5.
In our present work, the polysaccharides (MPS) were successfully extracted from the mycelia of Hypsizygus marmoreus, and purpose of our work was to evaluate the analysis of the characterization, anti-inflammation and anti-oxidation against the lung failure induced by lipopolysaccharide (LPS) in mice. Pretreatment with MPS at 800mg/kg markedly ameliorated the lung wet-to-dry weight (W/D) ratio and pulmonary histopathological conditions, reduced the tumor necrosis factor-alpha (TNF)-α, interleukin (IL)-6, and IL-1β levels in bronchoalveolar lavage fluid (BALF), myeloperoxidase (MPO) activities. In addition, the protective effects of MPS might be attributed to the down-regulations of the serum complement 3 (C3), glutamyl transpeptidase (GGT) and high-sensitivity C-reactive protein (hs-CPR) contents, as well as improving the antioxidant status by enhancing pulmonary enzyme activities (catalase (CAT), superoxide dismutase(SOD), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC)) and eliminating the lipid peroxidation (LPO) and malondialdehyde (MDA), respectively. What's more, our work investigated the characterization of the MPS.
在我们目前的工作中，成功地从杏鲍菇的菌丝体中提取了多糖（MPS），我们的工作目的是评估其特性分析、对脂多糖（LPS）诱导的小鼠肺衰竭的抗炎和抗氧化作用。用 800mg/kg 的 MPS 预处理可显著改善肺湿重/干重（W/D）比值和肺组织病理学状况，降低支气管肺泡灌洗液（BALF）中的肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-6 和 IL-1β 水平，髓过氧化物酶（MPO）活性。此外，MPS 的保护作用可能归因于下调血清补体 3（C3）、谷氨酰转肽酶（GGT）和高敏 C 反应蛋白（hs-CRP）含量，以及通过增强肺酶活性（过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和总抗氧化能力（T-AOC））和消除脂质过氧化（LPO）和丙二醛（MDA）来改善抗氧化状态。更重要的是，我们的工作还研究了 MPS 的特性。
