奥曲肽对多药耐药铜绿假单胞菌的协同和抗生物膜特性。
Synergistic and antibiofilm properties of ocellatin peptides against multidrug-resistant Pseudomonas aeruginosa.
机构信息
LAQV/REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto, 4169-007 Porto, Portugal.
Ciências Químicas e das Biomoléculas, Centro de Investigação em Saúde e Ambiente, Escola Superior de Saúde, Instituto Politécnico do Porto, 4200-072 Porto, Portugal.
出版信息
Future Microbiol. 2018 Feb;13:151-163. doi: 10.2217/fmb-2017-0175. Epub 2018 Jan 8.
AIM
To test ocellatin peptides (ocellatins-PT2-PT6) for antibacterial and antibiofilm activities and synergy with antibiotics against Pseudomonas aeruginosa.
MATERIALS & METHODS: Normal- and checkerboard-broth microdilution methods were used. Biofilm studies included microtiter plate-based assays and microscopic analysis by confocal laser scanning microscopy and atomic force microscopy.
RESULTS
Ocellatins were more active against multidrug-resistant isolates of P. aeruginosa than against susceptible strains. Ocellatin-PT3 showed synergy with ciprofloxacin and ceftazidime against multidrug-resistant isolates and was capable of preventing the proliferation of 48-h mature biofilms at concentrations ranging from 4 to 8× the MIC. Treated biofilms had low viability and were slightly more disaggregated.
CONCLUSION
Ocellatin-PT3 may be promising as a template for the development of novel antimicrobial peptides against P. aeruginosa. [Formula: see text].
目的
测试小眼章鱼肽（ocellatins-PT2-PT6）对铜绿假单胞菌的抗菌和抗生物膜活性及与抗生素的协同作用。
材料与方法
采用常规和棋盘微量稀释法。生物膜研究包括微孔板检测法和通过共聚焦激光扫描显微镜及原子力显微镜进行的微观分析。
结果
小眼章鱼肽对多重耐药性铜绿假单胞菌分离株的活性高于对敏感株的活性。ocellatin-PT3 与环丙沙星和头孢他啶对多重耐药性分离株表现出协同作用，并能在浓度为 MIC 的 4 至 8 倍时抑制 48 小时成熟生物膜的增殖。经处理的生物膜具有较低的存活率且略有分散。
结论
ocellatin-PT3 可能是开发针对铜绿假单胞菌的新型抗菌肽的有前途的模板。
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