Kim H A, Lee H S, Shin T H, Jung J Y, Baek W Y, Park H J, Lee G, Paik M J, Suh C H
1 Department of Rheumatology and BK21 Division of Cell Transformation and Restoration, 37977 Ajou University School of Medicine , Suwon, Republic of Korea.
2 College of Pharmacy, 65380 Sunchon National University , Suncheon, Republic of Korea.
Lupus. 2018 May;27(6):930-938. doi: 10.1177/0961203317751860. Epub 2018 Jan 7.
Systemic lupus erythematosus (SLE) is a systemic autoimmune disease with various clinical manifestations and serologic markers. In this study, we analyzed nine polyamine (PA) profiles of plasma from patients with SLE and healthy controls (HCs), and the relationship between the PA profiles and disease activity. PA alterations in plasma of 44 patients with SLE and fever were investigated using gas chromatography mass spectrometry (GC-MS) in selected ion monitoring mode using N-ethoxycarbonyl/ N-pentafluoropropionyl derivatives, and compared with those of 43 HCs. Patients with SLE and HCs showed differences in five of nine PA profiles. Among five changed PA levels, four PAs, namely N-acetylcadaverine, spermidine, N-acetylspermidine, and spermine, were dramatically decreased. However, the level of cadaverine was increased in patients with SLE. In the partial correlation with PA profiles and disease activity markers of SLE, several disease activity markers and nutritional markers were correlated with cadaverine, spermidine, and N -acetylspermidine. Thus, our results provide a comprehensive understanding of the relationship between PA metabolomics and disease activity markers in patients with SLE.
系统性红斑狼疮（SLE）是一种具有多种临床表现和血清学标志物的全身性自身免疫性疾病。在本研究中，我们分析了SLE患者和健康对照（HCs）血浆中的九种多胺（PA）谱，以及PA谱与疾病活动度之间的关系。使用气相色谱 - 质谱联用仪（GC-MS）在选择离子监测模式下，采用N-乙氧羰基/N-五氟丙酰基衍生物，对44例伴有发热的SLE患者血浆中的PA变化进行了研究，并与43例HCs的PA变化进行了比较。SLE患者和HCs在九种PA谱中的五种存在差异。在五种变化的PA水平中，四种PA，即N-乙酰尸胺、亚精胺、N-乙酰亚精胺和精胺，显著降低。然而，SLE患者尸胺水平升高。在SLE的PA谱与疾病活动度标志物的偏相关性分析中，几种疾病活动度标志物和营养标志物与尸胺、亚精胺和N-乙酰亚精胺相关。因此，我们的研究结果为全面了解SLE患者PA代谢组学与疾病活动度标志物之间的关系提供了依据。
