用于检测和鉴别耐万古霉素肠球菌种类的新型菌落多重聚合酶链反应检测法。
New colony multiplex PCR assays for the detection and discrimination of vancomycin-resistant enterococcal species.
作者信息
Nomura Takahiro, Hashimoto Yusuke, Kurushima Jun, Hirakawa Hidetada, Tanimoto Koichi, Zheng Bo, Ruan Genjie, Xue Feng, Liu Jian, Hisatsune Junzo, Sugai Motoyuki, Tomita Haruyoshi
机构信息
Department of Bacteriology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan.
Laboratory of Bacterial Drug Resistance, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan.
出版信息
J Microbiol Methods. 2018 Feb;145:69-72. doi: 10.1016/j.mimet.2017.12.013. Epub 2018 Jan 5.
New colony multiplex PCR assays for detection of seven types of vancomycin-resistance determinants and eight types of Enterococcus species were developed. For 135 enterococcal isolates examined in this study, these assays showed high sensitivity and specificity, and could provide the rapid and accurate detection of vancomycin-resistant determinants and Enterococcus spp.
开发了用于检测七种万古霉素耐药决定簇类型和八种肠球菌属物种的新型菌落多重PCR检测方法。对于本研究中检测的135株肠球菌分离株，这些检测方法显示出高灵敏度和特异性，并且能够快速准确地检测万古霉素耐药决定簇和肠球菌属。
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