Curtino J A, Chiodi J C
Biochem Int. 1985 Jul;11(1):107-15.
Bovine retina membrane proteins and glycoproteins were insoluble in chloroform/methanol (2:1, v/v) unless the membrane suspension was precipitated with trichloroacetic acid and the organic solvent mixture added to the precipitated membranes. The presence of millimolar amount of trichloroacetic acid in the organic solvent led to the total solubilization of membranes. The glycoproteins precipitated at the interphase after partition of the acidified chloroform/methanol solution with water and were resolubilized from the interphase with chloroform/methanol/water (1:1:0.3, by vol). The solubility properties of the membrane glycoproteins in the acidified organic solvent mixtures allow to remove the bulk of membrane lipids and to recover from the chloroform/methanol/water solution the glycoprotein of rod outer segment membranes, rhodopsin, as protonated N-retinylidene opsin in a water soluble form.
牛视网膜膜蛋白和糖蛋白不溶于氯仿/甲醇（2:1，v/v），除非膜悬浮液用三氯乙酸沉淀，然后将有机溶剂混合物加入沉淀的膜中。有机溶剂中存在毫摩尔量的三氯乙酸会导致膜完全溶解。用水分隔酸化的氯仿/甲醇溶液后，糖蛋白在界面处沉淀，然后用氯仿/甲醇/水（1:1:0.3，体积比）从界面处重新溶解。膜糖蛋白在酸化有机溶剂混合物中的溶解性特性使得能够去除大部分膜脂质，并从氯仿/甲醇/水溶液中回收视杆外段膜的糖蛋白视紫红质，其以水溶性形式的质子化N-视黄叉视蛋白存在。
